抗GATA3抗体 | Anti-GATA3 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human tonsil tissue using GTX54527 GATA3 antibody. Right : Primary antibodyLeft : Negative control without primary antibodyAntigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 minDilution : 1:200
	WB analysis of whole cell extracts (20 ug lysate) of SH-SY5Y (Lane 1), Jurkat (Lane 2) and K-562 (Lane 3) using GTX54527 GATA3 antibody. Dilution : 1:500-1:1500
	WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX54527 GATA3 antibody. Dilution : 1:1000
	WB analysis of 20 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX54527 GATA3 antibody. Dilution : 1:1000
	ICC/IF analysis of MCF7 (left panel) and SH-SY5Y cells (right panel) using GTX54527 GATA3 antibody. Green : Primary antibodyBlue : NucleiRed : ActinFixation : FormalinPermeabilization : 0.1% Triton X-100 in TBS for 10 minutesDilution : 1:50 for at least 1 hour at room temperature
	ICC/IF analysis of MCF-7 cells using GTX54527 GATA3 antibody. Panel e is a no primary antibody control.Green : Primary antibodyBlue : NucleiRed : ActinFixation : 4% paraformaldehydePermeabilization : 0.1% Triton„ X-100 for 10 minutesDilution : 1 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:1500
ICC/IF	1-2 μg/ml
IHC-P	1:50-1:500
FACS	3-5 μg/10⁶ cells

Not tested in other applications.

Calculated MW	48 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 30% glycerol, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	Purified partial recombinant GATA3 protein expressed in E. coli
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	GATA binding protein 3 , HDR , HDRS
Cellular Localization	Nucleus
Background	This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Database	・ Gene ID: 2625 GATA3 ・ UniProt: P23771 GATA3

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