抗B-Raf (V600E Mutant Specific) [RM8]抗体 | Anti-B-Raf (V600E Mutant Specific) [RM8] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.
	Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. WiDr Cells (400X, Positive)
	Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. SW40 Cells (400X, Negative)
	Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.
	Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of RM8 to the V600E mutation. His-tag fusion proteins were captured by an Anti-His-Tag antibody.

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製品情報

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM8
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity	Human

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APPLICATION

Application Note

Immunohistochemistry (IHC): 2 – 10 ug/mL, Immunocytochemistry (ICC): 2 – 10 ug/mL, Western Blot (WB): 0.5 – 2 ug/mL

Calculated MW	84 kDa. ( Note )
Specificity/Sensitivity	This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.

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PROPERTIES

Form	Liquid
Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	A peptide corresponding to BRAF V600E mutant
Purification	Protein A affinity purified from an animal origin-free culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	B-Raf Proto-Oncogene, Serine/Threonine Kinase , B-Raf1 , B-Raf , Braf1 , Ns7 , Rafb1 , Braf
Background	This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 673 ・ UniProt: P15056

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Hansen CH et al. Proc Natl Acad Sci U S A 2017; (Epub) Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection.	Application : ELISA

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