抗ROCK1抗体 | Anti-ROCK1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of mouse spleen tissue using GTX32843 ROCK1 antibody. Dilution : 1:100
	WB analysis of normal (control) and ROCK1 knockout (KO) HeLa cell lysate using GTX32843 ROCK1 antibody.Loading : 25ug per laneDilution : 1:1000
	WB analysis of various sample lysates using GTX32843 ROCK1 antibody.Loading : 25ug per laneDilution : 1:1000
	IP analysis of 293T cells using GTX32843 ROCK1 antibody.IP reaction : 200μg extracts / 3μg antibody antibodyDilution : 1:1000

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:2000
IHC-P	1:50-1:100
IP	1:50-1:100

Not tested in other applications.

Calculated MW	158 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH7.3, 0.02% sodium azide, 50% glycerol.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization	Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane
Background	This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]
Database	・ Gene ID: 6093 ROCK1 ・ UniProt: Q13464 ROCK1

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