抗ROCK1抗体 | Anti-ROCK1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31836 ROCK1 antibody. Working concentration : 1 μg/ml
	ICC/IF analysis of 293 cells using GTX31836 ROCK1 antibody. Working concentration : 10 μg/ml
	ICC/IF analysis of 293 cells using GTX31836 ROCK1 antibody. Working concentration : 20 μg/ml

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1 μg/mL
ICC/IF	10 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	158 kDa. ( Note )
Positive Control	HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)
Specificity/Sensitivity	ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1.The immunogen is located within the first 50 amino acids of ROCK1.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization	Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane
Background	This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 6093 ROCK1 ・ UniProt: Q13464 ROCK1

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