抗Bim抗体 | Anti-Bim antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of various cell lines using GTX31697 Bim antibody.Dilution : 5μg/mLLoading : 15μg of lysates per lane
	WB analysis of various samples using GTX31697 Bim antibody.Lane 1 : Human urinary bladder tissue lysateLane 2 : Human pancreas tissue lysateDilution : 5μg/mLLoading : 15μg of lysates per lane
	ICC/IF analysis of 4% paraformaldehyde-fixed K562 cells using GTX31697 Bim antibody.Dilution : 20μg/mL
	WB analysis of Daudi and HeLa cell lysate using GTX31697 Bim antibody.Dilution : 5μg/mLLoading : 15μg of lysates per lane
	ICC/IF analysis of formaldehyde-fixed K562 cells using GTX31697 Bim antibody.Antigen retrieval : Heat mediation with a citrate buffer (pH6)Dilution : 10μg/mL

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	5 μg/mL
ICC/IF	10 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	22 kDa. ( Note )
Positive Control	K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911)
Predict Reactivity	Rat(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim.The sequence is identical to that of mouse and rat.The immunogen is located within amino acids 80 - 130 of Bim.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	BCL2 like 11 , BAM , BIM , BOD
Background	The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 10018 BCL2L11 ・ UniProt: O43521 BCL2L11

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