抗Bcl-2抗体 | Anti-Bcl-2 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of 4% PFA-fixed Human Kidney Cells using GTX31686 Bcl-2 antibody.Dilution : 10μg/mL
	WB analysis of A-20 cell lysate using GTX31686 Bcl-2 antibody.
	IHC-P analysis of 4% PFA-fixed Mouse Brain Tissue using GTX31686 Bcl-2 antibody.Dilution : 20μg/mL
	IHC-P analysis of formaldehyde fixed Mouse Brain Tissue using GTX31686 Bcl-2 antibody.Antigen retreival : Heat mediation with a citrate buffer (pH6)Dilution : 5μg/mL
	WB analysis of MCF-7 and THP-1 cell lysate using GTX31686 Bcl-2 antibody.
	IHC-P analysis of formaldehyde fixed Human Kidney Tissue using GTX31686 Bcl-2 antibody.Antigen retreival : Heat mediation with a citrate buffer (pH6)Dilution : 2μg/mL
	WB analysis of various cell lines using GTX31686 Bcl-2 antibody.Dilution : 2μg/mLLoading : 15μg of lysates per lane

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2μg/mL
IHC-P	2 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	26 kDa. ( Note )
Positive Control	A20 Cell Lysate(GTX27180) , Human Kidney Tissue Lysate(GTX29995) , Human Kidney Tissue Slide
Predict Reactivity	Rat, Bovine, Chicken(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2.The immunogen is located within the first 50 amino acids of Bcl-2.
Purification	Purified by ion exchange chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	BCL2 apoptosis regulator , Bcl-2 , PPP1R50
Cellular Localization	Mitochondrion outer membrane,Endoplasmic reticulum membrane
Background	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 596 BCL2 ・ UniProt: P10415 BCL2

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