抗PD-L1抗体 | Anti-PD-L1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	FACS analysis of A-20 cells using GTX31308 PD-L1 antibody. Working concentration : 0.5 μg/mlGreen: Isotype controlRed : PD-L1 antibody
	IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody. Working concentration : 20 μg/ml
	IHC-P analysis of human tonsil tissue using GTX31308 PD-L1 antibody. Working concentration : 5 μg/ml
	IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody. Working concentration : 2.5 μg/ml
	WB analysis of Raji cell lysate using GTX31308 PD-L1 antibody. Working concentration : (A) 0.5 and (B) 1 μg/ml
	IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody. Red : Primary antibodyDilution : 1:250
	IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody. Antigen retrieval : Heat mediation with a citrate buffer (pH6)Dilution : 5 μg/ml
	WB analysis of various sample lysates using GTX31308 PD-L1 antibody. Loading : 15μg of lysates per laneDilution : 2 μg/ml
	WB analysis of PD-L1 overexpression in 293 cells using GTX31308 PD-L1 antibody. Lane 1 : non-transfected 293 cellsLane 2 : PD-L1 overexpressed 293 cellsLoading : 15μg of lysates per laneDilution : 1 μg/ml
	WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2) using GTX31308 PD-L1 antibody. Loading : 10μg of lysates per laneDilution : 2 μg/ml

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS, ELISA, IHC
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.5 - 1 μg/mL
IHC-P	2.5 μg/mL
FACS	0.5 μg/ml
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW	33 kDa. ( Note )
Positive Control	Raji Cell Lysate(GTX27908) , Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide
Specificity/Sensitivity	PD-L1 antibody has no cross-reactivity to PD-L2.

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	PD-L1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1.The immunogen is located within amino acids 60 - 110 of PD-L1(Accession number: NP_054862).
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1
Cellular Localization	Cell membrane
Database	・ Gene ID: 29126 CD274 ・ UniProt: Q9NZQ7 CD274

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wang LA et al. Tumour Biol 2015; The prediction of survival of patients with gastric cancer with PD-L1 expression using contrast-enhanced ultrasonography.	Application : IHC Reactivity : Human

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