抗LC3A [166AT1234]抗体 | Anti-LC3A [166AT1234] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of various samples using GTX17380 LC3A antibody [166AT1234]. Lane 1: human brain lysates Lane 2: mouse brain lysates Lane 3: rat brain lysatesDilution : 1:2000
	ICC/IF analysis of U251 cells untreatd (left) or treated with Chloroquine (50 μM,16h) (right) using GTX17380 LC3A antibody [166AT1234]. Green : LC3A Blue : DAPIDilution : 1:25
	ICC/IF analysis of U251 cells treated with Chloroquine (50 μM,16h) using GTX17380 LC3A antibody [166AT1234]. Green : LC3ABlue : cell nuclearFixation : 4% paraformaldehyde (PFA) (20 min)Permeabilization : Triton X-100 (0.2%, 30 min)Dilution : 1:200
	WB analysis of multiple cell lysate using GTX17380 LC3A antibody [166AT1234]. Lane 1: Y79 (soluble fraction of cell extract) Lane 2: 293 transfected with human LC3A (whole cell extract)Dilution : 1:1000

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	166AT1234
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000
ICC/IF	1:25-200
IHC-P	Assay dependent
IHC	Assay dependent

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.09% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	This LC3A antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Purification	Protein G purified, followed by dialysis against PBS.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Shih CH et al. Part Fibre Toxicol 2018; 15 (1) : 44 Chronic pulmonary exposure to traffic-related fine particulate matter causes brain impairment in adult rats.	Application : IHC Reactivity : Rat
Rabionet M et al. Hum Mol Genet. 2015; 24 (17):4792-808 Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
Vlahava VM et al. Cell Signal 2015; 27 (6):1253-63 CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism.
Hsin IL et al. Mol Pharm 2015; 12 (5):1534-43 gmi, an immunomodulatory protein from ganoderma microsporum, potentiates *-induced apoptosis via autophagy in lung cancer cells.	Reactivity : Human
He Z et al. Mol Med Rep 2015; 11 (6):914416 * induces autophagic cell death in prostate cancer cells in vitro.

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