抗HMGB1 [HAP46.5]抗体 | Anti-HMGB1 [HAP46.5] antibody

• 2025/11/04

  試薬 特別価格

  [GNT]一次抗体製品25％OFFキャンペーン[～2026/02/27]

  期間：2025/11/04 ～2026/02/27

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  GeneTex International Corporation（GNT）

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	HAP46.5
Isotype	IgG1
Application	WB, ELISA
Reactivity	Human, Mouse, Rat, Dog, Chicken

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.5-1 μg/ml
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	25 kDa. ( Note )
Positive Control	MCF7

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PROPERTIES

Form	Liquid
Buffer	0.01M PBS, 0.02% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	purified recombinant human HMG-1.
Purification	Purified immunoglobulin
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
Cellular Localization	Nucleus,Cytoplasm,Secreted,Cell membrane,Endosome
Background	HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25 kDa members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. Both HMG1 and HMG2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMG1/2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites, such as steroid hormone receptors, p53, and Oct. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication.
Database	・ Gene ID: 3146 HMGB1 ・ UniProt: P09429 HMGB1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Prasad R et al. Nucleic Acids Res 2012; 40 (22) : 11571-82 Pol β associated complex and base excision repair factors in mouse fibroblasts.
Yang J et al. J Cell Sci 2010; 123 (Pt 6) : 861-70 GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.	Application : WB Reactivity : Human
Sun L et al. Cardiovasc Res 2009; 82 (2) : 371-81 RAGE mediates oxidized LDL-induced pro-inflammatory effects and atherosclerosis in non-diabetic LDL receptor-deficient mice.	Application : WB Reactivity : Mouse

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