抗E-Cadherin [DECMA-1]抗体 | Anti-E-Cadherin [DECMA-1] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3,200. Cells were fixed and permeabilized with cold methanol followed by acetone.
	WB analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:250.
	ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3200. Cells were fixed and permeabilized with methanol followed by acetone.

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製品情報

Host	Rat
Clonality	Monoclonal
Clone Name	DECMA-1
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Human, Mouse, Bovine, Dog

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	1:1,600
IHC-P	Assay dependent
IHC-Fr	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW	98 kDa. ( Note )
Positive Control	MDCK
Specificity/Sensitivity	Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.

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PROPERTIES

Form	Liquid
Buffer	Ascites, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Mouse
Immunogen	mouse embryonal carcinoma cell line PCC4 Aza R1.
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	cadherin 1 , AA960649 , ARC-1 , E-cad , Ecad , L-CAM , UVO , Um
Cellular Localization	Cell junction,Cell membrane; Single-pass type I membrane protein,Endosome
Background	Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.
Database	・ Gene ID: 12550 Cdh1 ・ UniProt: P09803 Cdh1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Takagaki Y et al. Sci Rep 2019; 9 (1) : 7519 Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice.	Application : IHC-P Reactivity : Mouse
Hart KC et al. Proc Natl Acad Sci U S A 2017; 114 (29) : E5845-E5853 E-cadherin and LGN align epithelial cell divisions with tissue tension independently of cell shape.
Gloerich M et al. Nat Commun 2017; 8 : 13996 Cell division orientation is coupled to cell-cell adhesion by the E-cadherin/LGN complex.	Application : ICC/IF Reactivity : Dog
Cheng F et al. Proc Natl Acad Sci U S A 2016; 113 (30) : E4320-7 Vimentin coordinates fibroblast proliferation and keratinocyte differentiation in wound healing via TGF-β-Slug signaling.	Application : WB Reactivity : Mouse
Peppicelli S et al. Clin Exp Metastasis. 2014; 31 (4) : 423-33 Contribution of acidic melanoma cells undergoing epithelial-to-mesenchymal transition to aggressiveness of non-acidic melanoma cells.	Application : WB Reactivity : Mouse

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