抗MAP2 [HM-2]抗体 | Anti-MAP2 [HM-2] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	MAP2 antibody [HM-2] detects MAP2 protein at by immunofluorescent analysis.Sample: Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAP2 protein stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:250.Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.
	MAP2 antibody [HM-2] detects MAP2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: MAP2 protein stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:250.Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:500.
	MAP2 antibody [HM-2] detects MAP2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MAP2 antibody [HM-2] (GTX11267) diluted at 1:500.
	Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP2 antibody [HM-2] (GTX11267) diluted at 1:2000. The signal was developed with Trident ECL plus-Enhanced.
	MAP2 antibody [HM-2] detects MAP2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MAP2 antibody [HM-2] (GTX11267) diluted at 1:500.
	MAP2 antibody [HM-2] detects MAP2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: MAP2 protein stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
	WB analysis of rat brain extract using GTX11267 MAP2 antibody [HM-2]at 1 μg/mL.
	ICC/IF analysis of B35 cells using GTX11267 MAP2 antibody [HM-2] at 2 μg/mL(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	HM-2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity	Human, Mouse, Rat, Bovine, Chicken, Quail

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2 μg/ml
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW	202 kDa. ( Note )
Positive Control	rat brain
Specificity/Sensitivity	Monoclonal Anti-MAP2 antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.

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PROPERTIES

Form	Liquid
Buffer	0.01M PBS pH7.4, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	~2? mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Rat
Immunogen	rat brain microtubule-associated proteins (MAPs).
Purification	Purified immunoglobulin
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	microtubule-associated protein 2 , MAP2R , Mtap2
Cellular Localization	Cytoplasm, cytoskeleton,Cell projection, dendrite
Background	MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.
Database	・ Gene ID: 25595 Map2 ・ UniProt: P15146 Map2

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Vasquez V et al. J Alzheimers Dis 2018; 66 (2) : 453-460 An Inducible Alpha-Synuclein Expressing Neuronal Cell Line Model for Parkinson's Disease1.	Application : ICC/IF Reactivity : Human
Paillusson S et al. Acta Neuropathol 2017; (Epub) α-Synuclein binds to the ER-mitochondria tethering protein VAPB to disrupt Ca2+ homeostasis and mitochondrial ATP production.	Application : ICC/IF Reactivity : Human
Rivera-Ramirez N et al. Neurochem Int 2016; 101 : 38-47 Histamine H3 receptor activation stimulates calcium mobilization in a subpopulation of rat striatal neurons in primary culture, but not in synaptosomes.	Application : ICC/IF Reactivity : Rat
Kadri F et al. J Biol Chem 2015; 290 (52) : 30931-46 HIV-1-Tat Protein Inhibits SC35-mediated Tau Exon 10 Inclusion through Up-regulation of DYRK1A Kinase.	Application : ICC/IF Reactivity : Mouse
Chu YY et al. Mol Neurobiol 2015; (Epub) Astrocytic CCAAT/Enhancer Binding Protein δ Regulates Neuronal Viability and Spatial Learning Ability via miR-135a.	Application : IHC-Fr Reactivity : Mouse

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