抗PHD2抗体 | Anti-PHD2 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) and hypoxia-treated HeLa cells lysate using GTX10930 PHD2 antibody. Dilution : 1 μg/ml
	WB analysis of DDDDK-his-PHD1, PHD2, PHD3 recombinant protein, (10 ng/lane), hypoxia-treated HeLa and MEF cell lysate using GTX10930 PHD2 antibody.
	FACS analysis of 10⁶ Jurkat cells (fixed and permeabilized) in 150μl reaction using GTX10930 PHD2 antibody. Dilution : 3 μg/ml

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, IP, EM
Reactivity	Human, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2500
ICC/IF	Assay dependent
IHC-P	Assay dependent
FACS	1:10 - 1:1000
IP	Assay dependent
EM	Assay dependent

Not tested in other applications.

Calculated MW	46 kDa. ( Note )
Specificity/Sensitivity	This product does not react with Mouse.

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PROPERTIES

Form	Liquid
Buffer	Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.09% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide made to a C-terminal portion of human PHD2/HIF Prolyl Hydroxylase 2 (between amino acids 350-426) (GeneID 54583).
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	egl-9 family hypoxia inducible factor 1 , C1orf12 , ECYT3 , HALAH , HIF-PH2 , HIFPH2 , HPH-2 , HPH2 , PHD2 , SM20 , ZMYND6
Cellular Localization	Cytoplasm,Nucleus
Background	The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq]
Database	・ Gene ID: 54583 EGLN1 ・ UniProt: Q9GZT9 EGLN1

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