抗c-Met抗体 | Anti-c-Met antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, FACS, IHC, Neutralizing/Blocking
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.1-0.2 μg/ml
FACS	Assay dependent
IHC	5-15 μg/ml
Neutralizing/Blocking	0.5-2 μg/ml

Not tested in other applications.

Calculated MW	156 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS containing carbohydrates
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	purified recombinant human hepatocyte growth factor receptor extracellular domain expressed in mouse NSO cells.
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met
Cellular Localization	Membrane; Single-pass type I membrane protein
Background	c Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF). The mature c Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprised of an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.HGF/SF binding to c Met stimulates receptor dimerization and the phosphorylation of numerous residues within the receptor_x005F_x0019_s cytoplasmic domain. Signaling proteins that are phosphorylated and/or localized in response to c Met phosphorylation include: Grb2, Shc, Cbl, Crk, cortactin, paxillin, GAB1, PI3K, FAK, Src, Ras, ERK1 and 2, JNK, PLC gamma, AKT, and STAT3. HGF/SF stimulation of c Met expressing cells enhances proliferation, migration, morphogenesis, and protease synthesis, characteristics that are associated with invasive cell phenotype. Many types of cancer exhibit sustained c Met stimulation, overexpression, or mutation, including carcinomas of the colon, breast, ovary, lung, liver, prostate, thyroid, kidney, as well as melanomas and sarcomas. In addition to cancer studies, other research areas in which c Met is under investigation include organogenesis, organ regeneration, angiogenesis and surgical wound healing.The detection limit in immunoblotting for recombinant human HGF R is approximately 5 ng/lane under non-reducing and reducing conditions. Both the alpha and beta chains of HGF R are detected by this antibody under reducing conditions. The detection limit in ELISA for recombinant human HGF R is approximately 0.16 ng/well.
Database	・ Gene ID: 4233 MET ・ UniProt: P08581 MET

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