抗Caspase 8抗体 | Anti-Caspase 8 antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1,000

Not tested in other applications.

Calculated MW	55 kDa. ( Note )
Positive Control	Raji
Specificity/Sensitivity	By immunoblotting, the antibody reacts with caspase 8a (55 kDa) and caspase 8b (54 kDa) and weakly cross-reacts with MACHβ3/MACHβ4 isoforms (30-32 kDa).

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PROPERTIES

Form	Liquid
Buffer	0.01M PBS pH7.4, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	synthetic peptide corresponding to amino acids 209-224 of the human caspase 8a/MACHα1 precursor, conjugated to KLH. The sequence contains the N-terminal region of the enzyme p18 subunit and is identical in other human caspase 8 isoforms (caspase 8b/MACHα2, MACHβ3, MACHβ4) and highly conserved (>85%) in mouse caspase 8.
Purification	IgG fraction
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5
Cellular Localization	Cytoplasm
Background	Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase_x0013_9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.
Database	・ Gene ID: 841 CASP8 ・ UniProt: Q14790 CASP8

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