抗AKT1 [PKB-175]抗体 | Anti-AKT1 [PKB-175] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IP analysis of MCF-7 cells using GTX10438 AKT1 antibody [PKB-175]. Lane 1 : IP from MCF-7 cells using10 μL antibody Lane 2 : IP from MCF-7 cells using10 μL nonrelevant antibody Lane 3 : MCF-7 whole cell lysate
	WB analysis of (1)HeLa (2) HepG2 (3) A549 (4) HEK-293 (5) JURKAT (6) MCF-7 cells using GTX10438 AKT1 antibody [PKB-175] at 1:5,000.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	PKB-175
Isotype	IgG1
Application	WB, IP, ELISA
Reactivity	Human, Mouse, Rat, Bovine, Chicken

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5,000
IP	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	56 kDa. ( Note )
Positive Control	MCF7

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PROPERTIES

Form	Liquid
Buffer	Ascites, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Human
Immunogen	Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA
Cellular Localization	Cytoplasm,Nucleus,Cell membrane
Background	The Akt family of serine/threonine protein kinases (also called protein kinase B/PKB) plays a key role in insulin signaling, cellular survival, and transformation. Akt1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.
Database	・ Gene ID: 207 AKT1 ・ UniProt: P31749 AKT1

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