抗ERBB2, Mouse-Mono(UMAB33) UltraMAB抗体 | Anti-ERBB2, Mouse-Mono(UMAB33) UltraMAB Antibody

• 価格
• UltraMAB抗体について
• Product Data
• Reference Data
• Image

価格

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UltraMAB抗体について

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Product Data

Immunogen	Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
Clone Name	Clone UMAB33	Isotype	IgG2a
Species Reactivity	Human, Dog	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application	WB, IHC, IF, 10K-CHIP	Suggested Dilutions	WB:1:500, IHC:1:50, IF:1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography

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Reference Data

Target Name	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1
Alternative Name	CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Database Link	NP_004439
Function	This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq].
Related Pathway

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Image

WB Image	Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (UM500033, clone UMAB33, 1:2000).
WB Image	Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody (Clone UMAB33) at 1:500.
IHC Image	Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERBB2mouse monoclonal antibody. (Clone UMAB33, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image	Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) using anti-ERBB2mouse monoclonal antibody. (Clone UMAB33, dilution 1:100; heat-induced epito
IF Image	Anti-ERBB2 mouse monoclonal antibody (UM500033, clone UMAB33, 1:100) immunofluorescent staining of COLO205 cells.
IF Image	Anti-ERBB2 mouse monoclonal antibody (UM500033, clone UMAB33, 1:100) immunofluorescent staining of MCF7 cells.
10K-CHIP Image	OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody (Clone UMAB33). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 (Clone UMAB33) very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.

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