抗Human DC-SIGN/CD209抗体(Anti-Human DC-SIGN/CD209 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Source	Monoclonal Mouse IgG2B Clone # 120507
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human DC-SIGN Fc Chimera (Catalog # 161-DC)
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of human lymph node
Adhesion Blockade	The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of DC‑SIGN in Human DC‑SIGN Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC‑SIGN and DC‑SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Flow Cytometry
	Detection of DC‑SIGN in Human Monocyte Derived Dendritic Cells by Flow Cytometry. Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Immunocytochemistry
	DC-SIGN in Human Dendritic Cells. DC-SIGN was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs	30835 (Human); 170786 (Mouse)
Background	DC-SIGN/CD209
background_content	Background: DC-SIGN/CD209 Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

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Citations

1. Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?
   Authors: KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten
   Eur. J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release
   Sci Rep, 2016;6(0):36222.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


3. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
   PLoS ONE, 2016;11(11):e0167410.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration
   Cell Death Dis, 2016;7(9):e2367.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
   Sci Rep, 2016;6(0):29447.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


6. Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.
   Authors: El-Awady, Ahmed R, Miles, Brodie, Scisci, Elizabet, Kurago, Zoya B, Palani, Chithra, Arce, Roger M, Waller, Jennifer, Genco, Caroline, Slocum, Connie, Manning, Matthew, Schoenlein, Patricia, Cutler, Christop
   PLoS Pathog, 2015;10(2):e1004647.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates.
   Authors: Rennick L, de Vries R, Carsillo T, Lemon K, van Amerongen G, Ludlow M, Nguyen D, Yuksel S, Verburgh R, Haddock P, McQuaid S, Duprex W, de Swart R
   J Virol, 2015;89(4):2192-200.
   


8. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
   Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
   PLoS ONE, 2015;10(3):e0120948.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


9. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
   Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
   PLoS Pathog, 2014;10(12):e1004548.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


10. Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues.
    Authors: Ferguson D, Mattiuzzo G, Ham C, Stebbings R, Li B, Rose N, Mee E, Smith D, Page M, Cranage M, Almond N, Towers G, Berry N
    PLoS ONE, 2014;9(8):e104390.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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