抗Human IGF-I R抗体(Anti-Human IGF-I R antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I RGlu31-Asn932Accession # P08069
Source	Monoclonal Mouse IgG1 Clone # 33255
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IGF-I R in sandwich ELISAs and Western blots. Detects mouse IGF-I R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL‑9 R, and rhTGF-beta  RII.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	5-25 µg/mL	See below
Human IGF-I R Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Mouse IGF‑I R Antibody (Catalog #MAB391 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human IGF‑I R Biotinylated Antibody (Catalog #BAF391 )
ELISA Standard		Recombinant Human IGF-I R Protein, CF (Catalog #391-GR )
Neutralization	Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. At 11 µg/mL, this antibody will neutralize approximately 50-75% of the bioactivity due to 6 ng/mL Recombinant Human IGF‑I.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Human IGF‑I R by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, SK‑Mel‑28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IGF-I R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
Flow Cytometry	click image to view larger	Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry.MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human IGF‑I R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry	click image to view larger	IGF‑I R in Mouse Heart. IGF‑I R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF‑I R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Neutralization	click image to view larger	Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I R Antibody. Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse IGF-I R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I R	Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.
Long Name:	Insulin-like Growth Factor I Receptor
Entrez Gene IDs:	3480 (Human)
Alternate Names:	CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

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Related Research Areas

Adipocytokines

Cancer Biomarkers

Endocrine Regulation of Lipid Metabolism

Glucose Homeostasis

IGF Family

Metabolic Peptide Hormones and Regulators

Peptide Receptors on VSMC

Platelet Cytokine and Growth Factor Receptors

Receptor Tyrosine Kinases (RTKs)

Receptor Tyrosine Kinases (RTKs) in the Akt Pathway

Receptors in the Jak/STAT Pathway

IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer Authors: Y Neuzillet, E Chapeaubla, C Krucker, L De Koning, T Lebret, F Radvanyi, I Bernard-Pi BMC Cancer, 2017;17(1):636. Species: Human Sample Type: Whole Cells Application: Neut

Western Blot
	Detection of Human IGF‑I R by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, SK‑Mel‑28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IGF-I R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
Flow Cytometry
	Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human IGF‑I R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
	IGF‑I R in Mouse Heart. IGF‑I R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF‑I R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Neutralization
	Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I R Antibody. Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse IGF-I R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity.

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Related Product & Information

Long Name	Insulin-like Growth Factor I Receptor
Entrez Gene IDs	3480 (Human)
Background	IGF-I R
background_content	Background: IGF-I R Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

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Citations

1. IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
   Authors: Y Neuzillet, E Chapeaubla, C Krucker, L De Koning, T Lebret, F Radvanyi, I Bernard-Pi
   BMC Cancer, 2017;17(1):636.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes
   Authors: M Gschweitl, A Ulbricht, CA Barnes, RI Enchev, I Stoffel-St, N Meyer-Scha, J Huotari, Y Yamauchi, UF Greber, A Helenius, M Peter
   Elife, 2016;5(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs
   Authors: Helmout Modjtahedi
   Int. J. Oncol., 2016;49(5):1825-1838.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.
   Authors: Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez-Villasenor I, Heath P, Romero I, Ince P, Wharton S
   Mol Brain, 2015;8(0):51.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators.
   Authors: Suh H, Zhao M, Derico L, Choi N, Lee S
   J Neuroinflammation, 2013;10(0):37.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Frozen


6. Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro.
   Authors: Cardillo T, Trisal P, Arrojo R, Goldenberg D, Chang C
   BMC Cancer, 2013;13(0):170.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity.
   Authors: Jacques C, Holzenberger M, Mladenovic Z
   J. Biol. Chem., 2012;287(18):15100-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Expression of the IGF axis is decreased in local prostate cancer but enhanced after benign prostate epithelial differentiation and TGF-beta treatment.
   Authors: Massoner P, Ladurner Rennau M, Heidegger I, Kloss-Brandstatter A, Summerer M, Reichhart E, Schafer G, Klocker H
   Am. J. Pathol., 2011;179(6):2905-19.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes.
   Authors: Back K, Arnqvist HJ
   Growth Horm. IGF Res., 2009;19(2):101-11.
   Species: Human
   Sample Type: Cell Lysates
   Application: ELISA Development


10. Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases.
    Authors: Wang N, Fallavollita L, Nguyen L, Burnier J, Rafei M, Galipeau J, Yakar S, Brodt P
    Mol. Ther., 2009;17(7):1241-9.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development

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