抗Human CCL20/MIP-3 alpha抗体(Anti-Human CCL20/MIP-3 alpha antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL20/MIP-3 alpha Ala27-Met96 (Asn95Asp)Accession # P78556.1
Source	Monoclonal Mouse IgG1 Clone # 67310
Purification	Protein A or G purified from ascites
Specificity	Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL19, rhCXCL1, rhCCL16, rhCCL11, recombinant mouse CCL20/MIP-3 alpha, or recombinant rat CCL20/MIP-3 alpha is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Human CCL20/MIP-3 alpha Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CCL20/MIP‑3 alpha Antibody (Catalog #MAB360 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog #BAF360 )
ELISA Standard		Recombinant Human CCL20/MIP-3 alpha Protein (Catalog #360-MP )
Neutralization	Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Human CCL20/MIP‑3 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Recombinant Human CCL20/MIP‑3 alpha by Western Blot. Western blot shows 100 ng of Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP), Recombinant Mouse CCL20/MIP‑3 alpha (Catalog # 760-M3) and Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL20/MIP‑3 alpha at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization	click image to view larger	Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody.Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360‑MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP‑3 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB360). The ND50 is typically 1-5 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL20/MIP-3 alpha	CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

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References

Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
Hromas, R. et al. (1997) Blood 89:3315.
Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Entrez Gene IDs:	6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names:	beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Related Research Areas

CC Chemokines and Receptors

Chemokines and Receptors

Chemokines and Receptors in Angiogenesis

Macrophage Activation Markers

Molecules Secreted by VSMC

Regulatory T Cells (Tregs)

Th17 Cells

Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm Authors: B Soto, T Gallastegi, C Rodríguez, J Martínez-G, JR Escudero, L Vila, M Camacho Sci Rep, 2017;7(1):17331. Species: Human Sample Type: Whole Tissue Application: IHC-P

Western Blot
	Detection of Recombinant Human CCL20/MIP‑3 alpha by Western Blot. Western blot shows 100 ng of Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP), Recombinant Mouse CCL20/MIP‑3 alpha (Catalog # 760-M3) and Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL20/MIP‑3 alpha at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody. Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360‑MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP‑3 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB360). The ND50 is typically 1-5 µg/mL.

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Related Product & Information

Entrez Gene IDs	6364 (Human); 20297 (Mouse); 29538 (Rat)
Background	CCL20/MIP-3 alpha
background_content	Background: CCL20/MIP-3 alpha CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

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Citations

1. Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm
   Authors: B Soto, T Gallastegi, C Rodríguez, J Martínez-G, JR Escudero, L Vila, M Camacho
   Sci Rep, 2017;7(1):17331.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


2. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
   Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
   Autophagy, 2014;10(2):257-68.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
   Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
   Clin. Cancer Res., 2011;17(24):7529-38.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


5. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
   Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
   J. Pathol., 2008;214(3):328-36.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: ELISA Development


6. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
   Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
   J. Immunol., 2008;181(10):7420-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
   Authors: Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG
   Am. J. Pathol., 2008;173(1):195-204.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


8. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
   Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
   Blood, 2007;109(9):3649-57.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


9. Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
   Authors: Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC
   Clin. Exp. Immunol., 2007;150(3):567-75.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


10. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006;172(1):156-65.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development

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