抗Human VEGF R2/KDR抗体(Anti-Human VEGF R2/KDR antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1Ala20-Glu764Accession # P35968
Source	Monoclonal Mouse IgG1 Clone # 89106
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-50 ng/mL in the presence of 50 ng/mL Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera and 10 ng/mL Recombinant Human VEGF165.

Flow Cytometry
	Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk‑1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Neutralization
	VEGF R2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGF R2/KDR Antibody. Recombinant Human VEGF R2/KDR Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF165 (Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165 (10 ng/mL) activity elicited by Recombinant Human VEGF R2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGF R2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND50 is typically 10-50 ng/mL.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
Background	VEGF R2/KDR/Flk-1
background_content	Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

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Citations

1. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
   Authors: Gabriela Odent Grig
   J Tissue Eng Regen Med, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Identification of a Novel Pathogenic Germline KDR Variant in Melanoma
   Authors: IP Silva, A Salhi, KM Giles, M Vogelsang, SW Han, N Ismaili, KP Lui, EM Robinson, MA Wilson, RL Shapiro, A Pavlick, J Zhong, T Kirchhoff, I Osman
   Clin. Cancer Res., 2016;22(10):2377-85.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
   Authors: Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M
   PLoS ONE, 2014;9(4):e93965.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. MicroRNA-16 affects key functions of human endothelial progenitor cells.
   Authors: Goretti E, Rolland-Turner M, Leonard F, Zhang L, Wagner D, Devaux Y
   J Leukoc Biol, 2013;93(5):645-55.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
   Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
   Nature, 2011;475(7355):226-30.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.
   Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
   Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.
   Authors: Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
   Biochem. Biophys. Res. Commun., 2008;368(3):543-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
   Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
   Leuk. Res., 2008;32(8):1244-58.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
   Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
   Blood, 2007;109(4):1479-89.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007;25(8):1862-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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