抗Human CCL24/Eotaxin-2抗体(Anti-Human CCL24/Eotaxin-2 antibody)
掲載日情報:2021/01/28 現在Webページ番号:177310
Human CCL24/Eotaxin-2に対する抗体(Anti-Human CCL24/Eotaxin-2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年12月03日 16時15分現在]
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Anti-Human CCL24/Eotaxin-2 MAb (Clone 61016) |
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Anti-Human CCL24/Eotaxin-2 MAb (Clone 61016) |
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Anti-Human CCL24/Eotaxin-2 MAb (Clone 61016) |
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[在庫・価格 :2025年12月03日 16時15分現在]
Anti-Human CCL24/Eotaxin-2 MAb (Clone 61016)
文献数: 1
- 商品コード:MAB343-100
- メーカー:RSD
- 包装:100μg
- 価格:¥74,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | 別名:chemokine (C-C motif) ligand 24 クローン:61016 Genbank No: 6369 Protein Accession No: AAB51135 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CCL24/Eotaxin-2 MAb (Clone 61016)
文献数: 1
- 商品コード:MAB343-500
- メーカー:RSD
- 包装:500μg
- 価格:¥249,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | マッチドペア:Human CCL24/Eotaxin-2/MPIF-2 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF343,スタンダードとして#343-E2-025を用いる。 別名:chemokine (C-C motif) ligand 24 クローン:61016 Genbank No: 6369 Protein Accession No: AAB51135 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CCL24/Eotaxin-2 MAb (Clone 61016)
文献数: 1
- 商品コード:MAB343-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥25,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:chemokine (C-C motif) ligand 24 クローン:61016 Genbank No: 6369 Protein Accession No: AAB51135 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human CCL24/Eotaxin‑2/MPIF‑2Val27-Cys119Accession # AAB51135 |
| Source | Monoclonal Mouse IgG1 Clone # 61016 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects human CCL24/Eotaxin‑2/MPIF‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.04% cross-reactivity with recombinant human (rh) Eotaxin, rhMIP-1 alpha, and rhMCP-3 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 343-E2) |
| Human CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL24/Eotaxin‑2/MPIF‑2 Antibody (Catalog #MAB343 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog #BAF343 ) |
| ELISA Standard | Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein (Catalog #343-E2 ) | |
| Neutralization | Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 100 ng/mL Recombinant Human CCL24/Eotaxin‑2/MPIF‑2. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![]() click image to view larger | Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody.Recombinant Human CCL24/ Eotaxin‑2 (Catalog # 343‑E2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/ Eotaxin‑2 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL24/ Eotaxin‑2 Monoclonal Antibody (Catalog # MAB343). The ND50 is typically 0.2‑0.8 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL24/Eotaxin-2/MPIF-2 | Eotaxin-2, also named MPIF-2 and Ck beta 6, is a CC chemokine that is designated CCL24. Eotaxin-2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C‑terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin-2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP-3, CCL3/MIP-1 alpha, and CCL11/Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin-2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2, Eotaxin and CCL13/MCP-4 are mediated by the receptor CCR3. |
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References
| Forssmann, U. et al. (1997) J. Exp. Med. 185:2171. | |
| Patel, V.P. et al. (1997) J. Exp. Med. 185:1163. | |
| Entrez Gene IDs: | 6369 (Human); 56221 (Mouse) |
| Alternate Names: | CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6 |
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Related Research Areas
| Airway Inflammation and Remodeling | ||
| CC Chemokines and Receptors | ||
| Chemokines and Receptors | ||
| Chemokines and Receptors in Allergy and Asthma | ||
| Eosinophils | ||
| Macrophage Activation Markers | ||
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Related Product & Information
| Entrez Gene IDs | 6369 (Human); 56221 (Mouse) |
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| Background | CCL24/Eotaxin-2/MPIF-2 |
| background_content | Background: CCL24/Eotaxin-2/MPIF-2 Eotaxin-2, also named MPIF-2 and Ck beta 6, is a CC chemokine that is designated CCL24. Eotaxin-2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C‑terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin-2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP-3, CCL3/MIP-1 alpha, and CCL11/Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin-2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2, Eotaxin and CCL13/MCP-4 are mediated by the receptor CCR3. |
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Citations
- Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
Clin. Cancer Res., 2007;13(19):5719-28.
Species: Human
Sample Type: Tissue Homogenates
Application: ELISA Development
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![Neutralization CCL24/Eotaxin-2/MPIF-2 Antibody (61016) [Unconjugated]](https://resources.rndsystems.com/images/datasheets/antibody/CCL24_MAB343_Block_Neutralize_9156.jpg)
