抗Human CCL11/Eotaxin抗体(Anti-Human CCL11/Eotaxin antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL11/EotaxinGly24-Pro97Accession # P51671
Source	Monoclonal Mouse IgG1 Clone # 43911
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CCL11/Eotaxin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 14, 17, 19, 20, 21, 25, recombinant mouse CCL2, 3, 4, 5,6, 7, 9/10/MIP-1 gamma, 11, 21, or 25.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) under non-reducing conditions only
Immunohistochemistry	8-25 µg/mL	See below
Human CCL11/Eotaxin Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CCL11/Eotaxin Antibody (Catalog #MAB320 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CCL11/Eotaxin Biotinylated Antibody (Catalog #BAF320 )
ELISA Standard		Recombinant Human CCL11/Eotaxin Protein (Catalog #320-EO )
Neutralization	Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CCL11/Eotaxin.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunohistochemistry	click image to view larger	CCL11/Eotaxin in Human Skin. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry	click image to view larger	CCL11/Eotaxin in Human Colon. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization	click image to view larger	Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCL11/Eotaxin Antibody.Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND50 is typically 1-5 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL11/Eotaxin	CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

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References

Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
Choe, H. et al. (1996) Cell 85:1135.
Entrez Gene IDs:	6356 (Human); 20292 (Mouse)
Alternate Names:	C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

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Related Research Areas

Show

Adult Neurogenesis
Airway Inflammation and Remodeling
CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
Eosinophils
Macrophage Activation Markers
Molecules Secreted by VSMC
The ageing systemic milieu negatively regulates neurogenesis and cognitive function. Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T Nature, 2011;477(7362):90-4. Species: Mouse Sample Type: Whole Cells Application: Neut
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Excellent		ELISA	Human	Anonymous	12/06/2017

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Summary

Show

Application	ELISA
Sample Tested	EDTA Plasma
Species	Human
Enlarge	Excellent		ELISA	Human	Anonymous	11/16/2017			Image Details	ELISA: Human CCL11/Eotaxin Antibody [MAB320].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human

Immunohistochemistry
	CCL11/Eotaxin in Human Skin. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	CCL11/Eotaxin in Human Colon. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCL11/Eotaxin Antibody. Recombinant Human CCL11/Eotaxin (Catalog #320-EO) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND50 is typically 1-5 µg/mL.

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Related Product & Information

Entrez Gene IDs	6356 (Human); 20292 (Mouse)
Background	CCL11/Eotaxin
background_content	Background: CCL11/Eotaxin CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

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Citations

1. The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
   Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
   Nature, 2011;477(7362):90-4.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


2. Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
   Authors: Miyagaki T, Sugaya M, Fujita H
   J. Invest. Dermatol., 2010;130(9):2304-11.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
   Authors: Osei-Kumah A, Smith R, Clifton VL
   Cytokine, 2008;43(2):187-93.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


4. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
   Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
   J. Allergy Clin. Immunol., 2007;120(5):1118-25.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
   Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
   Clin. Immunol., 2006;120(2):220-31.
   Species: Human
   Sample Type: Blister Fluid
   Application: Neut


6. Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
   Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
   Parasitology, 2005;130(0):333-42.
   Species: Human
   Sample Type: Saliva
   Application: ELISA Development


7. The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts.
   Authors: Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N
   J. Allergy Clin. Immunol., 2005;117(1):103-10.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
   Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
   Pediatr. Res., 2000;47(5):592-7.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development

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