抗Human CCL8/MCP-2抗体(Anti-Human CCL8/MCP-2 antibody)
掲載日情報:2021/01/28 現在Webページ番号:177271
Human CCL8/MCP-2に対する抗体(Anti-Human CCL8/MCP-2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年12月03日 15時55分現在]
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Anti-Human CCL8/MCP-2 MAb (Clone 35509) |
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Anti-Human CCL8/MCP-2 MAb (Clone 35509) |
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Anti-Human CCL8/MCP-2 MAb (Clone 35509) |
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[在庫・価格 :2025年12月03日 15時55分現在]
Anti-Human CCL8/MCP-2 MAb (Clone 35509)
文献数: 5
- 商品コード:MAB281-100
- メーカー:RSD
- 包装:100μg
- 価格:¥78,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | 別名:C-C motif chemokine 8 クローン:35509 Genbank No: 6355 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CCL8/MCP-2 MAb (Clone 35509)
文献数: 5
- 商品コード:MAB281-500
- メーカー:RSD
- 包装:500μg
- 価格:¥259,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | マッチドペア:Human CCL8/MCP-2 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF281,スタンダードとして#281-CP-010を用いる。 別名:C-C motif chemokine 8 クローン:35509 Genbank No: 6355 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CCL8/MCP-2 MAb (Clone 35509)
文献数: 5
- 商品コード:MAB281-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥25,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:C-C motif chemokine 8 クローン:35509 Genbank No: 6355 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human CCL8/MCP-2 |
| Source | Monoclonal Mouse IgG1 Clone # 35509 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects human CCL8/MCP-2 in ELISAs and Western blots. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant human (rh) CCL15 and rmCCL3. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP) |
| Human CCL8/MCP-2 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL8/MCP‑2 Antibody (Catalog #MAB281 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL8/MCP‑2 Biotinylated Antibody (Catalog #BAF281 ) |
| ELISA Standard | Recombinant Human CCL8/MCP-2 Protein (Catalog #281-CP ) | |
| Neutralization | Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.25 µg/mL Recombinant Human CCL8/MCP‑2. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![]() click image to view larger | Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/MCP‑2 Antibody.Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP‑2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL8/MCP‑2 Monoclonal Antibody (Catalog # MAB281). The ND50 is typically 1-4 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL8/MCP-2 | CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5. | |
| Entrez Gene IDs: | 6355 (Human); 20307 (Mouse) | |
| Alternate Names: | C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8 | |
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Related Research Areas
| CC Chemokines and Receptors | ||||||||||
| Chemokines and Receptors | ||||||||||
| Chemokines and Receptors in Allergy and Asthma | ||||||||||
| Infectious Diseases | ||||||||||
| Inflammatory Mediators | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Molecules Secreted by VSMC | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
![]() Enlarge | Very Good | ELISA | Human | Anonymous | 11/20/2017 | Image Details |
![]() ELISA: Human CCL8/MCP-2 Antibody [MAB281]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Human |
| Other Experimental Details | This antibody was used as the capture in an ELISA with BAF281 as the detection antibody. |
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Related Product & Information
| Entrez Gene IDs | 6355 (Human); 20307 (Mouse) |
|---|---|
| Background | CCL8/MCP-2 |
| background_content | Background: CCL8/MCP-2 CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5. |
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Citations
- A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children.
Authors: Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I
Genes Immun, 2016;17(1):52-9.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Capture - Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
J Allergy Clin Immunol, 2014;133(4):1227-30.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Capture - Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
Authors: Struyf S, Proost P, Vandercappellen J
Eur. J. Immunol., 2009;39(3):843-57.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
Ann. Rheum. Dis., 2006;65(3):294-300.
Species: Human
Sample Type: Whole Tissue
Application: IHC Frozen - Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
Authors: Tay SS, McCormack A, Rose ML
Transplantation, 2004;78(7):987-94.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
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![Neutralization CCL8/MCP-2 Antibody (35509) [Unconjugated]](https://resources.rndsystems.com/images/datasheets/antibody/CCL8_MAB281_Block_Neutralize_9123.jpg)

