抗Human CCL8/MCP-2抗体(Anti-Human CCL8/MCP-2 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL8/MCP-2
Source	Monoclonal Mouse IgG1 Clone # 35509
Purification	Protein A or G purified from ascites
Specificity	Detects human CCL8/MCP-2 in ELISAs and Western blots. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant human (rh) CCL15 and rmCCL3.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)
Human CCL8/MCP-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CCL8/MCP‑2 Antibody (Catalog #MAB281 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CCL8/MCP‑2 Biotinylated Antibody (Catalog #BAF281 )
ELISA Standard		Recombinant Human CCL8/MCP-2 Protein (Catalog #281-CP )
Neutralization	Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.25 µg/mL Recombinant Human CCL8/MCP‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/MCP‑2 Antibody.Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP‑2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL8/MCP‑2 Monoclonal Antibody (Catalog # MAB281). The ND50 is typically 1-4 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL8/MCP-2	CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.
Entrez Gene IDs:	6355 (Human); 20307 (Mouse)
Alternate Names:	C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8

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Related Research Areas

Show

CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Infectious Diseases
Inflammatory Mediators
Macrophage Activation Markers
Molecules Secreted by VSMC
A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children. Authors: Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I Genes Immun, 2016;17(1):52-9. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Capture
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Very Good		ELISA	Human	Anonymous	11/20/2017			Image Details	ELISA: Human CCL8/MCP-2 Antibody [MAB281].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human
Other Experimental Details	This antibody was used as the capture in an ELISA with BAF281 as the detection antibody.

Neutralization

Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/MCP‑2 Antibody. Recombinant Human CCL8/MCP‑2 (Catalog #281-CP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP‑2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-HumanCCL8/MCP‑2 Monoclonal Antibody (Catalog # MAB281). The ND50 is typically 1-4 µg/mL.

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Related Product & Information

Entrez Gene IDs	6355 (Human); 20307 (Mouse)
Background	CCL8/MCP-2
background_content	Background: CCL8/MCP-2 CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

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Citations

1. A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children.
   Authors: Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I
   Genes Immun, 2016;17(1):52-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


2. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
   Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
   J Allergy Clin Immunol, 2014;133(4):1227-30.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
   Authors: Struyf S, Proost P, Vandercappellen J
   Eur. J. Immunol., 2009;39(3):843-57.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


4. Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
   Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
   Ann. Rheum. Dis., 2006;65(3):294-300.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
   Authors: Tay SS, McCormack A, Rose ML
   Transplantation, 2004;78(7):987-94.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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