抗Human CXCL1/GRO alpha抗体(Anti-Human CXCL1/GRO alpha antibody)
掲載日情報：2021/01/28 現在Webページ番号：177264
Human CXCL1/GRO alphaに対する抗体(Anti-Human CXCL1/GRO alpha )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human CXCL1/GRO alpha MAb (Clone 20326) |
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Anti-Human CXCL1/GRO alpha MAb (Clone 20326) |
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Anti-Human CXCL1/GRO alpha MAb (Clone 20326) |
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[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human CXCL1/GRO alpha MAb (Clone 20326)
文献数： 4
- 商品コード：MAB275-100
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | クローン：20326 Genbank No： 2919 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CXCL1/GRO alpha MAb (Clone 20326)
文献数： 4
- 商品コード：MAB275-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CXCL1/GROα サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF275，スタンダードとして#275-GR-010を用いる。 クローン：20326 Genbank No： 2919 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CXCL1/GRO alpha MAb (Clone 20326)
文献数： 4
- 商品コード：MAB275-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 クローン：20326 Genbank No： 2919 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human, Primate |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human CXCL1/GRO alpha /KC/CINC-1 |
| Source | Monoclonal Mouse IgG2B Clone # 20326 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In Western blots, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) CXCL2/GRO beta and rhCXCL3/GRO gamma and no cross-reactivity with recombinant rat (rr)CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhMIP-1 alpha, recombinant mouse (rm)MIP-1 alpha, rmMIP-1 beta, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 275-GR) |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Intracellular Staining by Flow Cytometry | 0.25 µg/106 cells | See below |
| Human/Primate CXCL1/GRO alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog #MAB275 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (Catalog #BAF275 ) |
| ELISA Standard | Recombinant Human CXCL1/GRO alpha Protein (Catalog #275-GR ) | |
| Neutralization | Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 10 ng/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Intracellular Staining by Flow Cytometry |
![Intracellular Staining by Flow Cytometry CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CXCL1_MAB275_Flow_Cytometry_19293.jpg) click image to view larger | Detection of CXCL1/GRO alpha /KC/CINC‑1 in Human PBMCs Treated with LPS and Monensin by Flow Cytometry.Human peripheral blood mononuclear cell (PBMCs) treated with 1 μg/mL LPS for 24 hours and 3 μM monensin for 2 hours was stained with Mouse Anti-Human/Primate CXCL1/GRO alpha / KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). |
| Neutralization |
![Neutralization CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CXCL1_MAB275_Block_Neutralize_9116.jpg) click image to view larger | Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody.Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Primate CXCL1/GRO alpha /KC/CINC‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275). The ND50 is typically 3-15 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CXCL1/GRO alpha/KC/CINC-1 | The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. In addition to the initially cloned GRO gene, now designated CXCL1, two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta, which shared 90% and 86% amino acid sequence homology, respectively, with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. It remains to be seen if a unique GRO receptor(s) also exist. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity. |
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| Entrez Gene IDs: | 2919 (Human); 14825 (Mouse); 81503 (Rat) | |
| Alternate Names: | CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha | |
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Related Research Areas
| Alpha Granule Soluble Molecules | ||||||||||
| Chemokines and Receptors in Allergy and Asthma | ||||||||||
| Chemokines and Receptors in Angiogenesis | ||||||||||
| CXC Chemokines and Receptors | ||||||||||
| Inflammatory Mediators | ||||||||||
| Macrophage Activation Markers | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | Block/Neutralize | Human | Anonymous | 04/26/2017 | Image Details |
 Block/Neutralize: Human/Primate CXCL1/GRO alpha /KC/CINC-1 Antibody [MAB275]. | |||
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Summary
| Application | Block/Neutralize |
| Sample Tested | Neutrophils |
| Species | Human |
| Other Experimental Details | The migration speed of human neutrophils was test after treatment after treatment with anti-IL-8 (R&D) or anti-CXCL-1 (R&D). |
| Intracellular Staining by Flow Cytometry | |
|---|---|
 | Detection of CXCL1/GRO alpha /KC/CINC‑1 in Human PBMCs Treated with LPS and Monensin by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) treated with 1 μg/mL LPS for 24 hours and3 μM monensin for 2 hours was stained with Mouse Anti-Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). |
| Neutralization | |
 | Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody. Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Primate CXCL1/GRO alpha /KC/CINC‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275). The ND50 is typically 3-15 µg/mL. |
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Related Product & Information
| Entrez Gene IDs | 2919 (Human); 14825 (Mouse); 81503 (Rat) |
|---|---|
| Background | CXCL1/GRO alpha/KC/CINC-1 |
| background_content | Background: CXCL1/GRO alpha/KC/CINC-1 The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. In addition to the initially cloned GRO gene, now designated CXCL1, two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta, which shared 90% and 86% amino acid sequence homology, respectively, with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. It remains to be seen if a unique GRO receptor(s) also exist. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- CXCL1 induces senescence of cancer-associated fibroblasts via autocrine loops in oral squamous cell carcinoma
Authors: EK Kim, S Moon, DK Kim, X Zhang, J Kim
PLoS ONE, 2018;13(1):e0188847.
Species: Human
Sample Type: Whole Cells
Application: Neut - Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
Authors: CM Henry, SJ Martin
Mol. Cell, 2017;65(4):715-729.e5.
Species: Human
Sample Type: Whole Cells
Application: ICC - Metronomic chemotherapy prevents therapy-induced stromal activation and induction of tumor-initiating cells
Authors: Kelvin K Tsai
J. Exp. Med., 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Neut - Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
Blood, 2012;120(18):3793-802.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - The expression and prognostic impact of CXC-chemokines in stage II and III colorectal cancer epithelial and stromal tissue.
Authors: Oladipo O, Conlon S, O'Grady A
Br. J. Cancer, 2011;104(3):480-7.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
J. Biol. Chem., 2011;286(4):2636-47.
Species: Human
Sample Type: Whole Cells
Application: ICC - Protein chip discovery of secreted proteins regulated by the phosphatidylinositol 3-kinase pathway in ovarian cancer cell lines.
Authors: Moscova M, Marsh DJ, Baxter RC
Cancer Res., 2006;66(3):1376-83.
Species: Human
Sample Type: Whole Cells
Application: Immunodepletion - IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005;175(9):6177-89.
Species: Human
Sample Type: Cell Culture Supernates
Application: Neut - Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells.
Authors: Hagiwara H, Mitsumata M, Yamane T
Circulation, 1998;98(23):2584-90.
Species: Human
Sample Type: Cell Lysates
Application: WB
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