抗Human IL-10 R alpha抗体(Anti-Human IL-10 R alpha antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha His22-Asn235Accession # Q13651.1
Source	Monoclonal Mouse IgG1 Clone # 37607
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL‑10 R alpha.

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Applications and Data

	Recommended Concentration	Sample
Neutralization	Measured by its ability to neutralize IL‑10 R alpha -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.

Neutralization

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R alpha Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 R alpha Monoclonal Antibody (Catalog # MAB274). The ND50 is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL).

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Related Product & Information

Long Name	Interleukin 10 Receptor alpha
Entrez Gene IDs	3587 (Human); 16154 (Mouse)
Background	IL-10 R alpha
background_content	Background: IL-10 R alpha IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10. Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

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Citations

1. Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages
   Sci Rep, 2016;6(0):31959.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


2. IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo
   Authors: Leonie Brockmann
   J. Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


3. Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms.
   Authors: von Lanzenauer S, Wolk K, Hoflich C, Kunz S, Grunberg B, Docke W, Reineke U, Asadullah K, Sterry W, Volk H, Sabat R
   Genes Immun, 2015;16(1):8-14.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. IFNgamma/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children.
   Authors: Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo M, Arinaitwe E, Briggs J, Greenhouse B, Tappero J, Kamya M, Dorsey G, Feeney M
   PLoS Pathog, 2014;10(1):e1003864.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
   Authors: Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M
   Eur J Immunol, 2014;44(6):1615-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
   Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
   Nat Commun, 2014;5(0):3199.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
   Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
   J. Virol., 2008;82(7):3736-50.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
   Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
   Blood, 2005;105(2):750-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Neut


9. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
   Authors: Chang WL, Baumgarth N, Yu D, Barry PA
   J. Virol., 2004;78(16):8720-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004;104(2):453-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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