抗Human CCL4/MIP-1 beta抗体(Anti-Human CCL4/MIP-1 beta antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Summary
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta Ala24-Asn92Accession # P13236
Source	Monoclonal Mouse IgG2B Clone # 24006
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, less than 4% cross-reactivity with recombinant human(rh) CCL3/MIP‑1 alpha and no cross-reactivity with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below
Human CCL4/MIP-1 beta Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CCL4/MIP‑1 beta Antibody (Catalog #MAB271 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CCL4/MIP‑1 beta Biotinylated Antibody (Catalog #BAF271 )
ELISA Standard		Recombinant Human CCL4/MIP-1 beta Protein (Catalog #271-BME )
Neutralization	Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL4/MIP‑1 beta.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody.Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP‑1 beta (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL4/MIP‑1 beta Monoclonal Antibody (Catalog # MAB271). The ND50 is typically 0.3-1.2 µg/mL.
Intracellular Staining by Flow Cytometry	click image to view larger	Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP‑1 beta Monoclonal Antibody (Catalog # MAB271) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL4/MIP-1 beta	CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.
Entrez Gene IDs:	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
Alternate Names:	ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

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Related Research Areas

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CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
Infectious Diseases
Inflammatory Mediators
Macrophage Activation Markers
Molecules Secreted by VSMC
Neuroinflammation
Regulatory T Cells (Tregs)
Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu PLoS Pathog., 2018;14(2):e1006888.
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		Block/Neutralize	Human	Anonymous	04/26/2017			Image Details	Block/Neutralize: Human CCL4/MIP-1 beta Antibody [MAB271].

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Summary

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Application	Block/Neutralize
Sample Tested	Peripheral blood neutrophils
Species	Human
Other Experimental Details	Neutrophil derived CCL4 was neutralized in co-culture with A375 tumor cells. This effect on the extravasation of A375 MA2 cells was assessed.
	Excellent		Affinity Purification	Dengue virus	Anonymous	04/25/2016

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Summary

Application	Affinity Purification
Sample Tested	HUVEC human umbilical vein endothelial cells
Species	Dengue virus

Neutralization
	Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody. Recombinant HumanCCL4/MIP‑1 beta (Catalog #271-BME) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCCL4/MIP‑1 beta (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-HumanCCL4/MIP‑1 beta Monoclonal Antibody (Catalog # MAB271). The ND50 is typically0.3-1.2 µg/mL.
Intracellular Staining by Flow Cytometry
	Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP‑1 beta Monoclonal Antibody (Catalog # MAB271) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
Background	CCL4/MIP-1 beta
background_content	Background: CCL4/MIP-1 beta CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

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Citations

1. Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection
   Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu
   PLoS Pathog., 2018;14(2):e1006888.
   


2. HIV-1-induced impairment of dendritic cell cross talk with gammadelta T lymphocytes.
   Authors: Cardone M, Ikeda K, Varano B, Gessani S, Conti L
   J Virol, 2015;89(9):4798-808.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer.
   Authors: Kageyama S, Ikeda H, Miyahara Y, Imai N, Ishihara M, Saito K, Sugino S, Ueda S, Ishikawa T, Kokura S, Naota H, Ohishi K, Shiraishi T, Inoue N, Tanabe M, Kidokoro T, Yoshioka H, Tomura D, Nukaya I, Mineno J, Takesako K, Katayama N, Shiku H
   Clin Cancer Res, 2015;21(10):2268-77.
   Species: Human
   Sample Type: Whole Cells
   Application: ELISpot Development


4. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


5. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
   Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
   Cancer Res., 2009;69(15):6249-55.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
   Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
   Clin. Immunol., 2008;128(1):57-65.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


7. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
   Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
   J. Pathol., 2008;214(3):328-36.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: ELISA Development


8. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


9. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


10. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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