抗Human IL-15抗体(Anti-Human IL-15 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Monoclonal Mouse IgG1 Clone # 34593
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-15 in Western blots. In Western blots, no cross-reactivity with recombinant human IL‑2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑15 (Catalog # 247-ILB)
Neutralization	Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.007-0.042 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑15.

Neutralization

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑15 Monoclonal Antibody (Catalog # MAB247). The ND50 is typically0.007-0.042 µg/mL.

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Related Product & Information

Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
Background	IL-15
background_content	Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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Citations

1. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
   Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
   J Biol Chem, 2015;290(37):22338-51.
   Species: Human
   Sample Type: Protein
   Application: Neut


2. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
   Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
   PLoS ONE, 2012;7(7):e40620.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha.
   Authors: Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
   Blood, 2011;117(15):4032-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
   Authors: Perrot I, Deauvieau F, Massacrier C
   J. Immunol., 2010;185(4):2080-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
   Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
   Arthritis Rheum., 2006;54(7):2096-108.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Cloning and expression of feline interleukin 15.
   Authors: Dean GA, Barger A, Lavoy A
   Cytokine, 2005;29(2):77-83.
   Species: Feline
   Sample Type: Whole Cells
   Application: ICC


7. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
   Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
   Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
   Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
   J. Immunol., 2004;173(2):1463-76.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
   Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
   J. Immunol., 2002;168(9):4326-32.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Regulatory activity of autocrine IL-10 on dendritic cell functions.
    Authors: Corinti S, la Sala A, Pastore S
    J. Immunol., 2001;166(7):4312-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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