抗Human LDL R抗体(Anti-Human LDL R antibody)
掲載日情報：2021/01/28 現在Webページ番号：177225
Human LDL Rに対する抗体(Anti-Human LDL R )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年11月03日 13時35分現在]
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Anti-Human LDL R MAb (Clone 472413) |
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Anti-Human LDL R MAb (Clone 472413) |
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Anti-Human LDL R MAb (Clone 472413) |
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[在庫・価格 ：2025年11月03日 13時35分現在]
Anti-Human LDL R MAb (Clone 472413)
文献数： 8
- 商品コード：MAB2148-100
- メーカー：RSD
- 包装：100μg
- 価格：¥83,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：FH クローン：472413 Genbank No： 3949 Protein Accession No： P01130 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human LDL R MAb (Clone 472413)
文献数： 8
- 商品コード：MAB2148-500
- メーカー：RSD
- 包装：500μg
- 価格：¥276,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human LDL R サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF2148，スタンダードとして#2148-LD-025を用いる。 別名：FH クローン：472413 Genbank No： 3949 Protein Accession No： P01130 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human LDL R MAb (Clone 472413)
文献数： 8
- 商品コード：MAB2148-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥28,000
- 在庫：1個
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：FH クローン：472413 Genbank No： 3949 Protein Accession No： P01130 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human LDL RAla22-Arg788Accession # P01130 |
| Source | Monoclonal Mouse IgG1 Clone # 472413 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human LDL R in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDL R, recombinant human LRP-5, or rmLRP-6 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human LDL R (Catalog # 2148-LD) under non-reducing conditions only |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| Immunoprecipitation | 25 µg/mL | Conditioned cell culture medium spiked with Recombinant Human LDL R (Catalog # 2148‑LD), see our available Western blot detection antibodies |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Human LDL R Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human LDL R Antibody (Catalog #MAB2148 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human LDL R Biotinylated Antibody (Catalog #BAF2148 ) |
| ELISA Standard | Recombinant Human LDL R Protein (Catalog #2148-LD ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Flow Cytometry |
![Flow Cytometry LDL R Antibody (472413) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/LDL_R_MAB2148_Flow_Cytometry_8364.jpg) click image to view larger | Detection of LDL R in HepG2 Human Cell Line by Flow Cytometry.HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDL R Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: LDL R | The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDL R is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120‑160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDL R is 51% aa identical to the extracellular region of human VLDL R, and 79% aa identical to the extracellular region of mouse LDL R. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDL R gene cause the autosomal dominant disorder, familial hypercholesterolemia (6). |
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References
| Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66. | |
| Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273. | |
| Yamamoto, T. et al. (1984) Cell 39:27. | |
| Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828. | |
| Defesche, J.C. (2004) Semin. Vasc. Med. 4:5. | |
| Varret, M. et al. (2008) Clin Genet. 73:1. | |
| Long Name: | Low Density Lipoprotein Receptor |
| Entrez Gene IDs: | 3949 (Human); 16835 (Mouse); 300438 (Rat) |
| Alternate Names: | FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor |
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Related Research Areas
| Lipid/Cholesterol Metabolism | ||
| Lipoprotein Receptors and Associated Proteins | ||
| Obesity | ||
| Scavenger Receptors | ||
| Transport of Amyloid beta Across the Blood-Brain Barrier | ||
| Uptake and Transport Molecules in VSMC | ||
| Uptake and Transport Molecules on Endothelial Cells | ||
| Uptake of Amyloid beta by Glia Cells | ||
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| Flow Cytometry | |
|---|---|
 | Detection of LDL R in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDL R Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). |
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Related Product & Information
| Background | LDL R |
|---|---|
| background_content | Background: LDL R The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDL R is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120‑160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDL R is 51% aa identical to the extracellular region of human VLDL R, and 79% aa identical to the extracellular region of mouse LDL R. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDL R gene cause the autosomal dominant disorder, familial hypercholesterolemia (6). |
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Citations
- A novel mechanism causing familial hypercholesterolemia: The proprotein convertase subtilisin/kexin type 9-resistant Arg410Ser LDL receptor mutation
Authors: Delia Susan-Resi
J. Biol. Chem, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Flow - Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
Authors: Elodie Weider
J Biol Chem, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: IHC - Not specified - Endophilin marks and controls a clathrin-independent endocytic pathway.
Authors: Boucrot E, Ferreira A, Almeida-Souza L, Debard S, Vallis Y, Howard G, Bertot L, Sauvonnet N, McMahon H
Nature, 2015;517(7535):460-5.
Application: Functional Assay - Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor.
Authors: Amirache F, Levy C, Costa C, Mangeot P, Torbett B, Wang C, Negre D, Cosset F, Verhoeyen E
Blood, 2014;123(9):1422-4.
Species: Human
Sample Type: Whole Cells
Application: Flow - Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
J Allergy Clin Immunol, 2014;133(4):1227-30.
Species: Human
Sample Type: Whole Cells
Application: Flow - Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
J Biol Chem, 2013;288(15):10805-18.
Species: Human
Sample Type: Cell Lysates
Application: ELISA Development - Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
Authors: Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG
J. Biol. Chem., 2009;284(42):28856-64.
Species: Human
Sample Type: Cell Lysates
Application: WB
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