抗Human B7-2/CD86抗体(Anti-Human B7-2/CD86 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86Ala23-His244Accession # P42081
Source	Monoclonal Mouse IgG1 Clone # 37301
Purification	Protein A or G purified from ascites
Specificity	Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	5 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Knockout Validated	B7-2/CD86 is specifically detected in Ramos human Burkitt's lymphoma parental cell line but is not detectable in B7-2/CD86 knockout Ramos cell line
Neutralization	Measured by its ability to neutralize B7‑2/CD86-induced IL-2 secretion in the Jurkat human acute T cell leukemia cell line. Freeman, G.J. et al. (1993) Science 262:909. The Neutralization Dose (ND50) is typically 0.5-2.5 μg/mL in the presence of 2 μg/mL Recombinant Human B7‑2/CD86 Fc Chimera.

Western Blot
	Detection of Human B7‑2/CD86 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7‑2/CD86 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of B7‑2/CD86 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Flow Cytometry
	Detection of B7-2/CD86 in Human Ramos Cell Line by Flow Cytometry. Human Ramos lymphoma cell line was stained with Mouse Anti-Human B7Formatting the figure legends (adjusted HTML for better linewrapping). Onlyapplies to the website insert.2/CD86 Monoclonal Antibody (Catalog # MAB141, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat Anti-Mouse IgG secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Knockout Validated
	Western Blot Shows Human B7-2/CD86 Specificity by Using Knockout Cell Line. Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2/CD86 knockout Ramos cell line (KO). PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7‑2/CD86 at approximately 74 kDa (as indicated) in the parental Ramos cell line, but is not detectable in knockout Ramos cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Knockout Validated
	B7-2/CD86 Specificity is Shown by Flow Cytometry in Knockout Cell Line. B7‑2/CD86 knockout Ramos human lymphoma cell line was stained with Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the B7‑2/CD86 knockout Ramos cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Neutralization
	Cell IL-2 Secretion Induced by B7‑2/CD86 and Neutralization by Human B7‑2/CD86 Antibody. Recombinant Human B7‑2/CD86 Fc Chimera induces IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine kit (Catalog # D2050). Under these conditions, IL-2 secretion elicited by B7‑2/CD86 is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141). The ND50 is typically 0.5-2.5 μg/mL.

目次に戻る

Related Product & Information

Entrez Gene IDs	942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)
Background	B7-2/CD86
background_content	Background: B7-2/CD86 B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

目次に戻る

Citations

1. Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2
   Authors: Xun Qu
   Cell. Mol. Immunol., 2016;13(5):615-27.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
   Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
   J Immunol Res, 2014;2014(0):593562.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


3. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
   Authors: Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z
   Cancer Res, 2012;72(22):5733-43.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fresh


4. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
   Blood, 2012;119(26):6268-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Critical role of lipid rafts in CD154-mediated T cell signaling.
   Authors: El Fakhry Y, Alturaihi H, Diallo D, Merhi Y, Mourad W
   Eur. J. Immunol., 2010;40(3):770-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
   Authors: Tso GH, Law HK, Tu W
   Stem Cells, 2010;28(5):939-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells.
   Authors: van der Vliet HJ, Wang R, Yue SC, Koon HB, Balk SP, Exley MA
   J. Immunol., 2008;180(11):7287-93.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
   Authors: Sakthivel P, Shively V, Kakoulidou M
   J. Intern. Med., 2007;261(4):399-407.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


9. Human plasma contains a soluble form of CD86 which is present at elevated levels in some leukaemia patients.
   Authors: Hock BD, Patton WN, Budhia S, Mannari D, Roberts P, McKenzie JL
   Leukemia, 2002;16(5):865-73.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development

目次に戻る