抗beta-III Tubulin (2E9)抗体 | Anti-beta-III Tubulin (2E9) Antibody

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	Western Blot: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of Tubulin Beta 3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysates.
	Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - IF Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (NBP1-42568, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
	Flow Cytometry: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of A549 cells using anti-Tubulin Beta 3 mAb (green) and negative control (purple).
	Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of PANC-1 cells using Tubulin Beta 3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
	Simple Western: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Simple Western lane view shows a specific band for Tubulin Beta 3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

Tubulin Beta 3 is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of Tubulin Beta 3 is almost exclusively neuron-specific.

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