抗Human CXCR4抗体(Anti-Human CXCR4 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	3T3 cells transfected with human CXCR4
Source	Monoclonal Mouse IgG2A Clone # 44708
Purification	Protein A or G purified from ascites
Specificity	Reacts specifically with human and non-human cells expressing human CXCR4 (fusin) as detected by flow cytometry. It will also react with cells expressing feline CXCR4 but not rat CXCR4. This antibody does not cross-react with other chemokine receptors.

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Applications and Data

	Recommended Concentration	Sample
Neutralization	Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 0.2-1.2 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Neutralization

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB171). The ND50 is typically 0.2-1.2 µg/mL.

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Related Product & Information

Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Background	CXCR4
background_content	Background: CXCR4 CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T cell tropic HIV‑1 and -2 viral entry into cells.

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Citations

1. Isolated metastasis of an EGFR-L858R-mutated NSCLC of the meninges: the potential impact of CXCL12/CXCR4 axis in EGFRmut NSCLC in diagnosis, follow-up and treatment
   Authors: F Lüke, R Blazquez, RF Yamaci, X Lu, B Pregler, S Hannus, K Menhart, D Hellwig, HJ Wester, S Kropf, D Heudobler, J Grosse, J Moosbauer, M Hutterer, P Hau, MJ Riemenschn, M Bayerlová, A Bleckmann, B Polzer, T Bei beta barth, CA Klein, T Pukrop
   Oncotarget, 2018;9(27):18844-18857.
   Species: Human
   Sample Type: Whole Cells
   Application: Fluorescence cross-correlation


2. The MRPS18-2 protein levels correlate with prostate tumor progression and it induces CXCR4-dependent migration of cancer cells
   Authors: M Mushtaq, L Jensen, S Davidsson, OV Grygoruk, O Andrén, V Kashuba, E Kashuba
   Sci Rep, 2018;8(1):2268.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


3. Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


4. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
   Authors: DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M
   Cytokine, 2014;69(1):47-55.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. ZAP-70 promotes the infiltration of malignant B-lymphocytes into the bone marrow by enhancing signaling and migration after CXCR4 stimulation.
   Authors: Calpe E, Purroy N, Carpio C, Abrisqueta P, Carabia J, Palacio C, Castellvi J, Crespo M, Bosch F
   PLoS ONE, 2013;8(12):e81221.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
   Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
   Eur. J. Immunol., 2008;38(11):3050-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium.
   Authors: Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW
   Cancer Res., 2007;67(9):4244-53.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus.
   Authors: Wald O, Pappo O, Safadi R, Dagan-Berger M, Beider K, Wald H, Franitza S, Weiss I, Avniel S, Boaz P, Hanna J, Zamir G, Eid A, Mandelboim O, Spengler U, Galun E, Peled A
   Eur. J. Immunol., 2004;34(4):1164-74.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion.
   Authors: Singh S, Singh UP, Grizzle WE, Lillard JW
   Lab. Invest., 2004;84(12):1666-76.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry.
    Authors: Hatse S, Princen K, Vermeire K, Gerlach LO, Rosenkilde MM, Schwartz TW, Bridger G, De Clercq E, Schols D
    FEBS Lett., 2003;546(2):300-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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