抗Human CXCR4抗体(Anti-Human CXCR4 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	3T3 cells transfected with human CXCR4
Source	Monoclonal Mouse IgG2B Clone # 44717
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Reacts specifically with human and non-human cells expressing human CXCR4 (fusin) as detected by flow cytometry. It will also react with cells expressing feline CXCR4 but not rat CXCR4. This antibody does not cross-react with other chemokine receptors.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	Jurkat human acute T cell leukemia cell line
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Neutralization

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB173). The ND50 is typically 1-5 µg/mL.

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Related Product & Information

Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Background	CXCR4
background_content	Background: CXCR4 CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.

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Citations

1. Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor
   Authors: R Desplantes, C Lévêque, B Muller, M Lotierzo, G Ferracci, M Popoff, M Seagar, R Mamoun, O El Far
   Sci Rep, 2017;7(1):1032.
   Species: N/A
   Sample Type: Exosomes
   Application: Surface Plasmon Resonance


2. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
   Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
   J Immunol, 2014;192(8):3805-15.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Novel role for NFAT3 in ERK-mediated regulation of CXCR4.
   Authors: Huang, Keven, Kiefer, Christin, Kamal, Adeela
   PLoS ONE, 2014;9(12):e115249.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Production of hepatocyte-like cells from human pluripotent stem cells.
   Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
   Nat Protoc, 2013;8(2):430-7.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
   Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
   Cancer Res., 2012;72(1):66-75.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. CXCR7 functions as a scavenger for CXCL12 and CXCL11.
   Authors: Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M
   PLoS ONE, 2010;5(2):e9175.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1.
   Authors: Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK
   Int. J. Cancer, 2010;126(6):1302-15.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment.
   Authors: Albanesi C, Scarponi C, Pallotta S, Daniele R, Bosisio D, Madonna S, Fortugno P, Gonzalvo-Feo S, Franssen JD, Parmentier M, De Pita O, Girolomoni G, Sozzani S
   J. Exp. Med., 2009;206(1):249-58.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


9. pVHL co-ordinately regulates CXCR4/CXCL12 and MMP2/MMP9 expression in human clear-cell renal cell carcinoma.
   Authors: Struckmann K, Mertz K, Steu S, Storz M, Staller P, Krek W, Schraml P, Moch H
   J. Pathol., 2008;214(4):464-71.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. CCL28 is increased in human Helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin A-secreting cells.
    Authors: Hansson M, Hermansson M, Svensson H, Elfvin A, Hansson LE, Johnsson E, Sjoling A, Quiding-Jarbrink M
    Infect. Immun., 2008;76(7):3304-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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