抗Human CCR2抗体(Anti-Human CCR2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NS0 mouse myeloma cell line transfected with human CCR2Met1-Leu360Accession # NP_001116868
Source	Monoclonal Mouse IgG2B Clone # 48607
Purification	Protein A or G purified from ascites
Specificity	Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of CCR2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
	CCR2 in Human Tonsil. CCR2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	729230 (Human); 12772 (Mouse); 60463 (Rat)
Background	CCR2
background_content	Background: CCR2 CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

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Citations

1. Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys-197 and by transmembrane protein aptamers that target it for lysosomal degradation
   Authors: LM Petti, SA Marlatt, Y Luo, EH Scheideman, A Shelar, D DiMaio
   J. Biol. Chem., 2018;0(0):.
   Application: Flow


2. Macrophage infiltration and genetic landscape of undifferentiated uterine sarcomas
   Authors: J Przybyl, M Kowalewska, A Quattrone, B Dewaele, V Vanspauwen, S Varma, S Vennam, AM Newman, M Swierniak, E Baku?a-Zal, JA Siedlecki, M Bidzinski, J Cools, M van de Rij, M Debiec-Ryc
   JCI Insight, 2017;2(11):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression
   Authors: G Sebastiani, G Ventriglia, A Stabilini, C Socci, C Morsiani, A Laurenzi, L Nigi, C Formichi, B Mfarrej, A Petrelli, G Fousteri, TM Brusko, F Dotta, M Battaglia
   Sci Rep, 2017;7(1):6897.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. CSF-1 regulates the function of monocytes in Crohn's disease patients in remission
   Authors: JC Nieto, C Zamora, E Cantó, E Garcia-Pla, J Gordillo, MA Ortiz, C Juárez, S Vidal
   Sci Rep, 2017;7(1):92.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
   Authors: Zaidoon Al-Jaderi
   Front Immunol, 2016;7(0):437.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Evidence for chemokine synergy during neutrophil migration in ARDS
   Thorax, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
   Authors: Williams D, Anastos K, Morgello S, Berman J
   J Leukoc Biol, 2015;97(2):401-12.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. PC3-secreted microprotein is a novel chemoattractant protein and functions as a high-affinity ligand for CC chemokine receptor 2.
   Authors: Pei X, Sun Q, Zhang Y, Wang P, Peng X, Guo C, Xu E, Zheng Y, Mo X, Ma J, Chen D, Zhang Y, Zhang Y, Song Q, Guo S, Shi T, Zhang Z, Ma D, Wang Y
   J Immunol, 2014;192(4):1878-86.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism.
   Authors: Chandrasekaran, Prabha, Moore, Victoria, Buckley, Monica, Spurrier, Joshua, Kehrl, John H, Venkatesan, Sundarar
   PLoS ONE, 2014;9(1):e86998.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
    Authors: Manley, Nathan C, Caso, Javier R, Works, Melissa, Cutler, Andrew B, Zemlyak, Ilona, Sun, Guohua, Munhoz, Carolina, Chang, Sydney, Sorrells, Shawn F, Ermini, Florian, Decker, Johannes, Bertrand, Anthony, Dinkel, Klaus M, Steinberg, Gary K, Sapolsky, Robert M
    PLoS ONE, 2013;8(4):e61789.
    Species: Rat
    Sample Type: Whole Cells
    Application: Flow

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