Wnt-3a組換え体(リコンビナント)タンパク質 | Recombinant Human Wnt-3a
掲載日情報：2015/04/03 現在Webページ番号：160028
R&D Systems社製の高品質なWnt-3aの組換え体タンパク質(Recombinant Human Wnt-3a)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月26日 17時35分現在]
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[在庫・価格 ：2024年04月26日 17時35分現在]
Recombinant Human Wnt-3a
文献数： 117
- 商品コード：5036-WN-500
- メーカー：RSD
- 包装：500μg
- 価格：ご照会ください
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
純度：>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.，由来動物：Human，M.W.：37.4 kDa，Genbank：89780，エンドトキシンレベル：<1.0 EU per 1 µg of the protein by the LAL method.，産生：CHO 別名：MGC119418 Genbank No： 89780 Protein Accession No： NP_149122 |
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| 保存条件 | -20℃ | 法規備考 | |||||
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| 製品記事 |
R&D Systems社 Wnt-3a関連製品 Wnt関連製品 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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バルク注文に関して
※バルク包装品も承ります。お気軽にお問合せ下さい。
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CF(Carrier-Free)とは？
R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。
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Product Details
- Source
- Chinese Hamster Ovary cell line, CHO-derived, Ser19-Lys352
- Accession
- NP_149122
- Purity
- >75%, by SDS-PAGE with silver staining.
- Predicted Molecular Mass
- 37.4 kDa
- SDS-PAGE
- 40 kDa, reducing conditions
- Activity
- Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.The ED50 for this effect is typically 5-25 ng/mL.Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.Optimal concentrations should be determined by each laboratory for each application.
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Additional Information
- Molecule Information
- Wnt-3a
- Aliases
- undefined
- Entrez Gene IDs
- 89780 (Human); 22416 (Mouse)
- Background
- Wnt-3a
- Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation.Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Drugging a stem cell compartment using Wnt3a protein as a therapeutic.
Carlson , Jeffery , Cheng , Du, Dhamdhere , Girija R, Fang , Mark Y, Helms , Jill A, Jiang , Jie, Lee , Katherin, Liu , Bo, Mulligan , Kimberle, Olveda , Rebecca , Ransom , Ryan C, Weis , William
PLoS ONE 2014 9:e83650
Species: Human
Protein Application: Bioassay
Cripto-1 enhances the canonical Wnt/??-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors.
Cell Signal 2013 25:178-89
Species: Human
Protein Application: Bioassay
Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
De Lamo J, Edukulla R, Ikeda Y, Khan Y, Kudva Y, Ohmine S, Sakuma T, Squillace K, Terzic A, Thatava T
Mol Ther 2013 21:228-39
Species: Human
Protein Application: Bioassay
IQGAP1 protein regulates nuclear localization of beta-catenin via importin-beta5 protein in Wnt signaling.
Adachi S, Goto T, Iemura S, Natsume T, Sato A, Shibuya H
J Biol Chem 2013 288:36351-60
Species: Human
Protein Application: cell culture
Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo.
Bruin J, Fox J, Kieffer T, Narayan K, O'Neil J, Rezania A, Xu J
Stem Cells 2013 31:2432-42
Species: Human
Protein Application: Bioassay
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Burton L, Chan E, Fridlyand J, Koeppen H, Li J, Lin E, Merchant M, Moffat J, Neve R, Peng J, Penuel E, Ribas A, Settleman J, Sosman J, Sutherlin DP, Wang Y, Wilson TR, Yan Y
Nature 2012 487:505-9
Species: Human
Protein Application: Bioassay
Derivation of cerebellar neurons from human pluripotent stem cells.
Bhattacharya S, Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M
Curr Protoc Stem Cell Biol 2012 20:1H.51
The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
Frank , Mark Bar, Ichii , Michiko, Iozzo , Renato V, Kincade , Paul W
Blood 2012 119:1683-92
Species: Human
Protein Application: Bioassay
The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems.
Aad , Pauline , Echternkamp , Sherrill, Schreiber , Nicole B, Spicer , Leon J, Sypherd , David D
Biol Reprod 2012 87:79
Species: Bovine
Protein Application: Bioassay
Wnt5a and Wnt11 are essential for second heart field progenitor development.
Cohen E, Miller M, Moon R, Morrisey E, Wang Z
Development 2012 139:1931-40
Species: Mouse
Protein Application: Bioassay
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