抗Human IL-27 Ra/WSX-1/TCCR Phycoerythrin抗体(Anti-Human IL-27 Ra/WSX-1/TCCR Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL-27 R alpha /WSX‑1/TCCRGly34-Lys516Accession # Q6UWB1
Source	Monoclonal Mouse IgG2B Clone # 191106
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-27 R alpha /WSX‑1/TCCR in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human gp130 or  recombinant mouse IL-27 Ra is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of IL‑27 R alpha /WSX‑1/TCCR in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IL‑27 R alpha /WSX‑1/TCCR PE‑conjugated Monoclonal Antibody (Catalog # FAB14791P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

IL-27 R alpha/WSX-1/TCCR

background_content

Background: IL-27 R alpha/WSX-1/TCCR IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 96‑100 kDa member of the type I, group 2 cytokine receptor family (1 - 6). Mature IL‑27 R alpha  is a type I transmembrane glycoprotein that contains a 484 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 99 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a "box‑1" motif that may be involved with Janus kinases (3). One potential alternate splice form has been hypothesized that involves a 58 aa addition to the cytoplasmic domain and, based on mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 257‑636 may also occur in human (3, 7). The human IL‑27 R alpha extracellular region shares 63% amino acid identity with the mouse IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha /WSX‑1‑/‑ mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4+ and CD8+, and which express the IL‑27 receptor, can be induced by IL‑27 to form a double‑positive CD25+ FoxP3‑ IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

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Citations

1. IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations
   Authors: F Xu, J Yi, Z Wang, Y Hu, C Han, Q Xue, X Zhang, X Luan
   Immunol. Res., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice
   J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-gamma release by autologous NK cells.
   Authors: Morandi F, Airoldi I, Pistoia V
   J Immunol Res, 2014;2014(0):938561.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. A soluble form of IL-27Ralpha is a natural IL-27 antagonist.
   Authors: Dietrich C, Candon S, Ruemmele F, Devergne O
   J Immunol, 2014;192(11):5382-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
   Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
   J Immunol, 2014;192(6):2634-42.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
   Authors: Tzeng, Tsai-Ten, Tsay, Huey-Jen, Chang, Luping, Hsu, Chia-Lin, Lai, Tzu-Hsua, Huang, Fong-Lee, Shiao, Young-Ji
   J Biomed Sci, 2013;20(0):90.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer.
   Authors: Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, Wang G, Walser T, Krysan K, Sharma S, Dubinett S, Lee J
   J Exp Clin Cancer Res, 2013;32(0):97.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway.
   Authors: Chen, Qian, Swaminathan, Sanjay, Yang, De, Dai, Lue, Sui, Hongyan, Yang, Jun, Hornung, Ronald L, Wang, Yanmei, Huang, Da Wei, Hu, Xiaojun, Lempicki, Richard, Imamichi, Tomozumi
   PLoS ONE, 2013;8(3):e59194.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. IL-27-Induced Gene Expression Is Downregulated in HIV-Infected Subjects.
   Authors: Guzzo C, Hopman W, Che Mat N, Wobeser W, Gee K
   PLoS ONE, 2012;7(9):e45706.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2012;26(6):1365-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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