抗Human IL-18/IL-1F4 Propeptide Phycoerythrin抗体(Anti-Human IL-18/IL-1F4 Propeptide Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant human IL‑18/IL‑1F4Accession # Q14116
Source	Monoclonal Mouse IgG1 Clone # 74801
Purification	Protein A or G purified from ascites
Specificity	Detects the pro region of human IL-18/IL-1F4 in direct ELISAs and Western blots. Does not cross‑react with recombinant human IL-18, recombinant mouse IL-18 or recombinant rat IL-18.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑18/IL‑1F4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (upper panel) or treated with LPS for 24 hours (lower panel) were stained with Mouse Anti-Human IL‑18/IL‑1F4 PE‑conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Interleukin 18
Background	IL-18/IL-1F4
background_content	Background: IL-18/IL-1F4 Pro-IL-18 (pro-Interleukin 18; also known as pro-IGIF and pro-IL-1 gamma ) is a 24 kDa member of the IL-1 family of molecules. It is widely expressed, being produced by keratinocytes, intestinal epithelium, T cells, macrophages and osteoblasts. Human Pro-IL-18 is 193 amino acids (aa) in length. Although mature IL-18 induces IFN-gamma secretion by NK and T cells, Pro-IL-18 appears to have little intrinsic activity. Generally, active IL-18 is considered to arise from caspase-1 cleavage of Pro-IL-18 between Asp36-Tyr37. This generates an 18 kDa mature C-terminal fragment, and a 4 kDa (predicted) N-terminal prosegment that runs at 6 kDa in SDS-PAGE. Other proteases are known to process Pro-IL-18. Caspase-3 cleavage after Asp68 generates an inactive 14 kDa mature segment, Merpin beta -subunit cleavage after Asn52 generates a marginally active 17 kDa mature segment, while parasite Cys protease cleavage after Val47 generates an inactive 17 kDa mature molecule. One splice variant shows a deletion of aa 27-30. Over aa 2-36, human Pro-IL-18 shares 63% aa identity with mouse Pro-IL-18.

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Citations

1. Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells
   Authors: Y Hu, J Wang, H Zhang, H Xie, W Song, Q Jiang, N Zhao, S He
   Mediators Inflamm., 2017;2017(0):3090782.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
   Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
   J. Cell. Mol. Med., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Human CD8(+) EMRA T cells display a senescence-associated secretory phenotype regulated by p38 MAPK
   Authors: LA Callender, EC Carroll, RWJ Beal, ES Chambers, S Nourshargh, AN Akbar, SM Henson
   Aging Cell, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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