Recombinant Human Noggin

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価格

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製品詳細

name	Recombinant Human Noggin
Source	HEK293 cells
Synonyms	None
AA Sequence (monomer)	QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
Purity	≥ 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity	Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
Description	Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β family, and regulate their activity by inhibiting their access to signaling receptors.  The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule.  Noggin was originally identified as a BMP-4 antagonist whose action was critical for proper formation of the head and other dorsal structures.  Consequently, noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14.  Targeted deletion of Noggin in mice results in prenatal death, and a recessive phenotype displaying a severely malformed skeletal system.  Conversely, transgenic mice over-expressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.  Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer consisting of two 205 amino acid polypeptide chains.  Monomeric glycosylated noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions.
Cross Reactivity	Chicken, Dog, Human, Leech, Mouse, Rat

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画像

Recombinant Human Noggin Biological Activity Graph

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文献

1. First Author:Rada-Iglesias, A
   Title:A unique chromatin signature uncovers early developmental enhancers in humans.
   Citation:Nature; 470(7333):279-83.
   
2. First Author:Shetty, P
   Title:Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells.
   Citation:Asian Journal of Transfusion Science; 4(1):14-24.
   
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   Title:Parkinson's disease induced pluripotent stem cells with triplication of the a-synuclein locus.
   Citation:Nature Communications; 2:440
   
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   Title:Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation.
   Citation:Blood; 117(18):4915-23
   
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   Title:Recurrent genomic instability of chromosome 1q in neural derivatives of human embryonic stem cells.
   Citation:The Journal of Clinical Investigation; 122(2):569-74
   
6. First Author:Kang, K
   Title:NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells.
   Citation:Immune Network; 11(6):348-57.
   
7. First Author:Nefzger, C M
   Title:Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells.
   Citation:Stem Cells; 30(7):1349-61.
   
8. First Author:Das, I
   Title:Glucocorticoids alleviate intestinal ER stress by enhancing protein folding and degradation of misfolded proteins
   Citation:The Journal of Experimental Medicine; 210(6):1201-16.
   
9. First Author:Azzolin, L
   Title:YAP/TAZ Incorporation in the β-Catenin Destruction Complex Orchestrates the Wnt Response
   Citation:Cell; 158(1):157-70
   
10. First Author:Richter, A
    Title:BMP4 promotes EMT and mesodermal commitment in human embryonic stem cells via SLUG and MSX2.
    Citation:Stem Cells; 32(3):636-48
    

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