抗FOXP3, C-term抗体 | Anti-FOXP3, C-term antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	ICC/IF analysis of PFA fixed U2OS cells using GTX89934 FOXP3 antibody, C-term. Green : Primary antibodyBlue : DAPINegative control : Unimmunized goat IgGPermeabilization : 0.15% TritonDilution : 10μg/ml
	FACS analysis of PFA fixed Jurkat cells using GTX89934 FOXP3 antibody, C-term. Blue : Primary antibodyBlack : Isotype controlPermeabilization : 0.5% TritonDilution : 10μg/ml
	ICC/IF analysis of CD25-sorted (Treg) human blood cells using GTX89934 FOXP3 antibody, C-term. A: Primary antibody and FITC conjigated secondary antibody, B: Phase contrast.
	WB analysis of human muscle (A), Molt-4 (B) and negative control pancreas (C) lysate using GTX89934 FOXP3 antibody, C-term. Dilution : 1μg/mlLoading : 35μg protein in RIPA buffer
	IHC-P analysis of human tonsil using GTX89934 FOXP3 antibody, C-term. Antigen retrieval : Tris/EDTA buffer pH 9Dilution : 1μg/ml A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.

目次に戻る

製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-3μg/ml
ICC/IF	2-4μg/ml
IHC-P	Assay dependent
FACS	Assay dependent
Note : ICC/IF Strong expression of the protein seen in the nucleoplasm of U2OS cells.

Not tested in other applications.

Calculated MW	47 kDa. ( Note )
Positive Control	The peptide used to generate this antibody is available for purchase (GTX89934-PEP).
Specificity/Sensitivity	This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

目次に戻る

PROPERTIES

Form	Liquid
Buffer	Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2; NP_001107849.1.
Purification	Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	Forkhead Box P3 , Aiid , Dieter , Ipex , Jm2 , Pidx , Xpid , Foxp3
Background	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 50943 ・ UniProt: Q9BZS1

目次に戻る