抗alpha Smooth Muscle Actin, N-term抗体 | Anti-alpha Smooth Muscle Actin, N-term antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	The ICC/IF analysis of alpha Smooth Muscle Actin antibody, N-term was published by Minutoli L and colleagues in the journal J Biomed Sci in 2014 . PMID: 24606563
	ICC/IF analysis of PFA fixed HepG2 cells using GTX89701 alpha Smooth Muscle Actin antibody, N-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10μg/ml
	WB analysis of human (A) and rat (B) duodenum lysate using GTX89701 alpha Smooth Muscle Actin antibody, N-term. Dilution : 0.1-0.5μg/ml Loading : 35μg protein in RIPA buffer
	IHC-P analysis of human lung using GTX89701 alpha Smooth Muscle Actin antibody, N-term. Antigen retrieval : citrate buffer pH 6 Dilution : 3.5μg/ml
	WB analysis of HeLa (A) and NIH3T3 (B) cell lysate (35μg protein in RIPA buffer) using GTX89701 alpha Smooth Muscle Actin antibody, N-term. Dilution : 0.3μg/ml (HeLa) and 1μg/ml (NIH3T3)
	WB analysis of human (A) and mouse (B) heart lysate (35μg protein in RIPA buffer) using GTX89701 alpha Smooth Muscle Actin antibody, N-term. Dilution : 0.1μg/ml (human heart) and 0.3μg/ml (mouse heart)

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.1-0.5μg/ml
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW	42 kDa. ( Note )
Positive Control	The peptide used to generate this antibody is available for purchase (GTX89701-PEP).
Predict Reactivity	Dog(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1; NP_001135417.1.
Purification	Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Actin, Alpha 2, Smooth Muscle, Aorta , Actsa , Acta2
Background	The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Database	・ Gene ID: 59 ・ UniProt: P62736

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REFERENCE

Application Reference	Application/Reactivity
Zhang P et al. Biochem Biophys Res Commun 2017; (Epub) Ischemic flap survival improvement by composition-selective fat grafting with novel adipose tissue derived product - stromal vascular fraction gel.	Application : IHC-P Reactivity : Mouse
Herrnberger L et al. PLoS One 2014; 9 (12) : e115005 Formation of fenestrae in murine liver sinusoids depends on plasmalemma vesicle-associated protein and is required for lipoprotein passage.	Application : IHC-P Reactivity : Mouse
Minutoli L et al. J Biomed Sci 2014; 21 (1) : 19 Inhibitors of apoptosis proteins in experimental benign prostatic hyperplasia: effects of serenoa repens, selenium and lycopene.	Application : ICC/IF Reactivity : Rat

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