抗Talin-1, Internal抗体 | Anti-Talin-1, Internal antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

WB analysis of human peripheral mononucleocytes lysate using GTX88357 Talin-1 antibody, Internal. Dilution : 1μg/mlLoading : 35μg protein in RIPA buffer

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-3μg/ml
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW	270 kDa. ( Note )
Positive Control	The peptide used to generate this antibody is available for purchase (GTX88357-PEP).
Specificity/Sensitivity	This antibody is not expected to cross-react with the highly similar protein Talin 2.
Predict Reactivity	Mouse, Rat, Bovine, Dog(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide with sequence C-QEALHTQMLTAVQE, from the internal region of the protein sequence according to NP_006280.3.
Purification	Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Talin 1 , Ilweq , Tln , Talin-1 , Tln1
Cellular Localization	Colocalizes with LAYN at the membrane ruffles.
Background	This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]
Database	・ Gene ID: 7094 ・ UniProt: Q9Y490

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Beaty BT et al. J Cell Biol 2014; (Epub) Talin regulates moesin-NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis.	Application : WB, ICC/IF, IP Reactivity : Human

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