抗DAG1, Internal抗体 | Anti-DAG1, Internal antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of human skeletal muscle lysate using GTX88089 DAG1 antibody, Internal. Dilution : 0.2μg/mlLoading : 35μg protein in RIPA buffer
	WB analysis of mouse and rat skeletal muscle lysate using GTX88089 DAG1 antibody, Internal. Dilution : 0.1μg/mlLoading : 35μg protein in RIPA buffer

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.1-0.3μg/ml

Not tested in other applications.

Calculated MW	97 kDa. ( Note )
Positive Control	The peptide used to generate this antibody is available for purchase (GTX88089-PEP).
Specificity/Sensitivity	This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
Predict Reactivity	Bovine, Dog(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide with sequence C-HVGKHEYFMHATDK, from the internal region of the protein sequence according to NP_004384.4.
Purification	Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Dystroglycan 1 , 156Dag , A3A , Agrnr , Dag , Mddga9 , Mddgc7 , Mddgc9 , Dag1
Cellular Localization	The monomeric form translocates to the nucleus via the action of importins and depends on RAN. Nuclear transport is inhibited by Tyr-892 phosphorylation. In skeletal muscle, this phosphorylated form locates to a vesicular internal membrane compartment. In
Background	Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010]
Database	・ Gene ID: 1605 ・ UniProt: Q14118

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