抗beta Tubulin 3/ Tuj1抗体 | Anti-beta Tubulin 3/ Tuj1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of paraformaldehyde-fixed (4%) neonatal mouse brain cultures using GTX85469 beta Tubulin 3 / Tuj1 antibody. Green : Primary antibody RED : GFAP antibody BLUE : DAPI Dilution : 1:1000
	WB analysis of adult mouse brain lysate using GTX85469 beta Tubulin 3 / Tuj1 antibody.Dilution : 1:3000
	ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody. Green : Primary antibody RED : Actin antibody BLUE : DAPI Dilution : 1:1000
	ICC/IF analysis of paraformaldehyde-fixed (4%) SpH64 cells using GTX85469 beta Tubulin 3 / Tuj1 antibody. Green : Primary antibody RED : Actin antibody BLUE : DAPI Dilution : 1:1000

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製品情報

Host	Chicken
Clonality	Polyclonal
Isotype	IgY
Application	WB, ICC/IF, IHC
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:10000-1:20000
ICC/IF	Assay dependent
IHC	1:2000-1:5000

Not tested in other applications.

Calculated MW	50 kDa. ( Note )
Specificity/Sensitivity	tubulin, beta 3

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PROPERTIES

Form	Liquid
Buffer	PBS + sodium azide (0.02%, w/v)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Tubulin Beta 3 Class Iii , Cdcbm , Cdcbm1 , Cfeom3 , Cfeom3A , Feom3 , Tubb4 , Beta-4 , Tubb3
Cellular Localization	Cytoplasmic
Background	Human beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.
Database	・ Gene ID: 10381 ・ UniProt: Q13509

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Santarriaga S et al. Mol Cell 2018; 71 (2) : 216-228.e7 SRCP1 Conveys Resistance to Polyglutamine Aggregation.	Application : ICC/IF
Hauser M et al. Cell Rep 2018; 24 (6) : 1512-1522 The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage.	Application : ICC/IF Reactivity : Rat
Patitucci TN et al. Hum Mol Genet. 2015; (Epub) SMN deficiency does not induce oxidative stress in SMA iPSC-derived astrocytes or motor neurons.	Application : ICC/IF Reactivity : Human

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