抗Bora抗体 | Anti-Bora antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of mouse brain tissue using GTX85293 Bora antibody. Working concentration : 20 μg/ml
	WB analysis of Jurkat cell lysate using GTX85293 Bora antibody. Working concentration : (A) 1 and (B) 2 μg/ml
	IHC-P analysis of mouse brain tissue using GTX85293 Bora antibody. Working concentration : 2.5 μg/ml

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1 - 2 μg/mL
IHC-P	2.5 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	61 kDa. ( Note )
Positive Control	Jurkat Cell Lysate(GTX27899)
Specificity/Sensitivity	This antibody is specific for Bora N-Terminus

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Bora antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human Bora.The immunogen is located within amino acids 70 - 120 of Bora.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	BORA aurora kinase A activator , C13orf34
Background	Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
Database	・ Gene ID: 79866 BORA ・ UniProt: Q6PGQ7 BORA

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