抗GITRL抗体 | Anti-GITRL antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	ICC/IF analysis of THP-1 cells using GTX85076 GITRL antibody. Working concentration : 10 μg/ml
	WB analysis of (A) 5 ng, (B) 25 ng, and (C) 50 ng purified recombinant GITRL protein using GTX85076 GITRL antibody. Working concentration : 1 μg/ml

目次に戻る

製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Mouse

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1 μg/mL
ICC/IF	10 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	23 kDa. ( Note )
Positive Control	THP-1 Cell Slide(GTX25540) , THP-1 Cell Lysate(GTX27913)

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	GITRL antibody was raised against purified recombinant human GITR ligand.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	TNF superfamily member 18 , AITRL , GITRL , TL6 , TNLG2A , hGITRL
Cellular Localization	Cell membrane
Background	The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-kappaB and various mitogen-activated protein kinases (reviewed in 1). The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions (2,3). Its ligand GITRL is expressed in endothelial and antigen-presenting cells (4) and can activate NF-kappaB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR (5). Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappaB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2 (4).
Database	・ Gene ID: 8995 TNFSF18 ・ UniProt: Q9UNG2 TNFSF18

目次に戻る