抗alpha Actinin [7A4]抗体 | Anti-alpha Actinin [7A4] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of HEK293T cells transfected with alpha Actinin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84959 alpha Actinin antibody [7A4].Loading : 5 ug per lane
	ICC/IF analysis of HeLa cells transiently transfected with alpha Actinin plasmid using GTX84959 alpha Actinin antibody [7A4].
	IHC-P analysis of human ovary tissue using GTX84959 alpha Actinin antibody [7A4].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	IHC-P analysis of human bladder tissue using GTX84959 alpha Actinin antibody [7A4].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	WB analysis of various cell lines using GTX84959 alpha Actinin antibody [7A4].Loading : 10 ug per laneDilution : 1:200
	WB analysis of various samples using GTX84959 alpha Actinin antibody [7A4].Loading : 10 ug per laneDilution : 1:200
	IHC-P analysis of human breast tissue using GTX84959 alpha Actinin antibody [7A4].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	ICC/IF analysis of HeLa cells using GTX84959 alpha Actinin antibody [7A4].
	IHC-P analysis of human liver tissue using GTX84959 alpha Actinin antibody [7A4].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	IHC-P analysis of human kidney tissue using GTX84959 alpha Actinin antibody [7A4].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	WB analysis of wild type and alpha Actinin knock out HeLa cell lysate(10μg per lane) using GTX84959 alpha Actinin antibody [7A4].Dilution : 1:500

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	7A4
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Monkey

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW	103 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.
Purification	Purified by affinity chromatography From tissue culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	actinin alpha 1 , BDPLT15
Cellular Localization	Cytoplasm, cytoskeleton,Cell membrane,Cell junction
Background	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Database	・ Gene ID: 87 ACTN1 ・ UniProt: P12814 ACTN1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Rojas C et al. Br J Pharmacol 2019; (Epub) A novel and potent brain penetrant inhibitor of extracellular vesicle release.	Application : WB Reactivity : Human
Foertsch S et al. Brain Behav Immun 2019; (Epub) Sensory contact to the stressor prevents recovery from structural and functional heart damage following psychosocial trauma.	Application : IHC-P Reactivity : Mouse
Liang C et al. J Appl Physiol (1985) 2019; 126 (4) : 799-809 Functional and biochemical responses of skeletal muscle following a moderate degree of systemic iron loading in mice.	Application : WB Reactivity : Mouse

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