抗Survivin [2D9]抗体 | Anti-Survivin [2D9] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of human tissues (Lane 1-Testis ; Lane 2-Uterus ; Lane 3-Breast ; Lane 4-Brain ; Lane 5-Liver ; Lane 6-Ovary ; Lane 7-Thyroid gland ; Lane 8-colon) using GTX84824 Survivin antibody [2D9].Loading : 10 ug per laneDilution : 1:200
	IHC-P analysis of human kidney tissue using GTX84824 Survivin antibody [2D9].Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120ºC for 3min.Dilution : 1:50
	IHC-P analysis of human liver tissue using GTX84824 Survivin antibody [2D9].Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120ºC for 3min.Dilution : 1:50
	WB analysis of HEK293T cells transfected with Survivin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84824 Survivin antibody [2D9].Loading : 5 ug per lane
	ICC/IF analysis of COS7 cells transiently transfected with Survivin plasmid using GTX84824 Survivin antibody [2D9].
	FACS analysis of HEK293T cells transfected with either Survivin plasmid(Red) or empty vector control plasmid(Blue) using GTX84824 Survivin antibody [2D9].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	2D9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:2000
ICC/IF	1:100
IHC-P	Assay dependent
FACS	1:100

Not tested in other applications.

Calculated MW	16 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Full length human recombinant protein of human BIRC5 (NP_001159) produced in HEK293T cell.
Purification	Purified by affinity chromatography From tissue culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	baculoviral IAP repeat containing 5 , API4 , EPR-1
Cellular Localization	Cytoplasm,Nucleus
Background	Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Database	・ Gene ID: 332 BIRC5 ・ UniProt: O15392 BIRC5

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