抗PLK1 [8C12]抗体 | Anti-PLK1 [8C12] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of HEK293T cells transfected with PLK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83866 PLK1 antibody [8C12].Loading : 5 ug per laneDilution : 1:500
	ICC/IF analysis of COS7 cells transiently transfected with PLK1 plasmid using GTX83866 PLK1 antibody [8C12].Dilution : 1:100
	IP analysis of DDDDK tagged PLK1 overexpressed HEK293T lysate using GTX83866 PLK1 antibody [8C12].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.IP reaction : 2μg antibody / 500μl cell lysate
	FACS analysis of HEK293T cells transfected with either PLK1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83866 PLK1 antibody [8C12].Dilution : 1:100
	WB analysis of various cell lines using GTX83866 PLK1 antibody [8C12].Loading : 35 ug per laneDilution : 1:500

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	8C12
Isotype	IgG1
Application	WB, ICC/IF, FACS, IP
Reactivity	Human, Dog, Monkey

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2000
ICC/IF	1:100
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW	68 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Full-length protein expressed in 293T cell transfected with human PLK1 expression vector
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	polo like kinase 1 , PLK , STPK13
Cellular Localization	Nucleus
Background	Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
Database	・ Gene ID: 5347 PLK1 ・ UniProt: P53350 PLK1

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