抗STAT1 [4H9]抗体 | Anti-STAT1 [4H9] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of HEK293T cells transfected with STAT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83552 STAT1 antibody [4H9].Loading : 5 ug per lane
	ICC/IF analysis of COS7 cells transiently transfected with STAT1 plasmid using GTX83552 STAT1 antibody [4H9].
	IHC-P analysis of human endometrium tissue using GTX83552 STAT1 antibody [4H9].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	WB analysis of various cell lines using GTX83552 STAT1 antibody [4H9].Loading : 35 ug per lane
	ICC/IF analysis of HT29 cells using GTX83552 STAT1 antibody [4H9].
	IHC-P analysis of human kidney tissue using GTX83552 STAT1 antibody [4H9].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83552 STAT1 antibody [4H9].Loading : 10 ug per laneDilution : 1:200
	IHC-P analysis of human thyroid carcinoma tissue using GTX83552 STAT1 antibody [4H9].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	IHC-P analysis of human breast tissue using GTX83552 STAT1 antibody [4H9].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	4H9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Dog, Monkey

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:2000
ICC/IF	1:50-1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW	87 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Full length human recombinant protein of human STAT1 (NP_009330) produced in HEK293T cell.
Purification	Purified by affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF-3 , STAT91
Cellular Localization	Cytoplasm,Nucleus
Background	Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Database	・ Gene ID: 6772 STAT1 ・ UniProt: P42224 STAT1

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