抗VEGF [4E3]抗体 | Anti-VEGF [4E3] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human breast tissue using GTX83426 VEGF antibody [4E3].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	WB analysis of various cell lines using GTX83426 VEGF antibody [4E3].Loading : 10 ug per laneDilution : 1:200
	IHC-P analysis of human breast adenocarcinoma tissue using GTX83426 VEGF antibody [4E3].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	IHC-P analysis of human liver tissue using GTX83426 VEGF antibody [4E3].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	IHC-P analysis of human pancreas tissue using GTX83426 VEGF antibody [4E3].Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
	WB analysis of HEK293T cells transfected with VEGF plasmid (Right) or empty vector (Left) for 48 hrs using GTX83426 VEGF antibody [4E3].Loading : 5 ug per lane
	ICC/IF analysis of COS7 cells transiently transfected with VEGF plasmid using GTX83426 VEGF antibody [4E3].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	4E3
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW	27 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.
Purification	Purified by affinity chromatography From tissue culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	vascular endothelial growth factor A , MVCD1 , VEGF , VPF
Cellular Localization	Secreted
Background	Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Database	・ Gene ID: 7422 VEGFA ・ UniProt: P15692 VEGFA

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