抗LAG3, Internal抗体 | Anti-LAG3, Internal antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	FACS analysis of Jurkat cells using GTX81605 LAG3 antibody, Internal. Red : LAG3 Blue : Isotype controlFixation : 2% paraformaldehyde (PFA) (10 min)Permeabilization : 90% methanol (10 min)Dilution : 1:25
	WB analysis of various samples using GTX81605 LAG3 antibody, Internal. Lane 1: 293T/17 whole cell lysate Lane 2: mouse heart lysateLoading : 20 μg per laneDilution : 1:2000
	WB analysis of 293T/17 whole cell lysate using GTX81605 LAG3 antibody, Internal. Loading : 20 μg per laneDilution : 1:2000
	IHC-P analysis of human thymus tissue using GTX81605 LAG3 antibody, Internal. Antigen retrieval : heat-induced antigen retrieval with citrate buffer (pH6)Dilution : 1:25
	WB analysis of various samples using GTX81605 LAG3 antibody, Internal. Lane 1: human ovary lysate Lane 2: mouse liver lysateLoading : 20 μg per laneDilution : 1:1000
	WB analysis of various samples using GTX81605 LAG3 antibody, Internal. Lane 1: K562 whole cell lysates Lane 2: human liver lysates Lane 3: human ovary lysatesLoading : 20 μg per laneDilution : 1:2000
	WB analysis of mouse heart lysate using GTX81605 LAG3 antibody, Internal. Loading : 20 μg per laneDilution : 1:2000

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000
IHC-P	1:10-1:50
FACS	1:25

Not tested in other applications.

Calculated MW	57 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.09% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.
Purification	Protein A purified, followed by peptide affinity purification.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	lymphocyte activating 3 , CD223
Cellular Localization	Membrane; Single-pass type I membrane protein
Background	Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 3902 LAG3 ・ UniProt: P18627 LAG3

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